Differential roles of integrins alpha2beta1 and alphaIIbbeta3 in collagen and CRP-induced platelet activation

Platelets
Gavin E JarvisS P Watson

Abstract

Collagen and collagen-related peptide (CRP) activate platelets by interacting with glycoprotein (GP)VI. In addition, collagen binds to integrin alpha2beta1 and possibly to other receptors. In this study, we have compared the role of integrins alpha2beta1 and alphaIIbbeta3 in platelet activation induced by collagen and CRP. Inhibitors of ADP and thromboxane A2 (TxA2) substantially attenuated collagen-induced platelet aggregation and dense granule release, whereas CRP-induced responses were only partially inhibited. Under these conditions, a proportion of platelets adhered to the collagen fibres resulting in dense granule release and alphaIIbbeta3 activation. This adhesion was substantially mediated by alpha2beta1. The alphaIIbbeta3 antagonist lotrafiban potentiated CRP-induced dense granule release, suggesting that alphaIIbbeta3 outside-in signalling may attenuate GPVI signals. By contrast, lotrafiban inhibited collagen-induced dense granule release. These results emphasise the differential roles of alpha2beta1 and alphaIIbbeta3 in platelet activation induced by collagen and CRP. Further, they show that although ADP and TxA2 greatly facilitate collagen-induced platelet activation, collagen can induce full activation of those pla...Continue Reading

References

Aug 1, 1975·Archives of Surgery·J C Rosenberg, T L Sell
May 31, 1988·Biochemical and Biophysical Research Communications·S A SantoroV L Woods
Apr 15, 1986·Thrombosis Research·N G ArdlieL K Bell
Jan 1, 1986·Methods in Enzymology·A LundinA Pousette
Nov 1, 1968·The Journal of Experimental Medicine·G EvansE A Murphy
Feb 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·M B Zucker, J Peterson
Sep 15, 1996·Archives of Biochemistry and Biophysics·E PadoinM G Doni
Nov 14, 1996·European Journal of Pharmacology·G E JarvisM F Heath
Aug 1, 1997·The Journal of Clinical Investigation·T M ChiangA H Kang
Sep 20, 1997·Nature·S OffermannsM I Simon
Oct 17, 1998·Current Opinion in Hematology·M J BarnesR W Farndale
Feb 29, 2000·British Journal of Pharmacology·G E JarvisP Leff
Feb 7, 2001·The Journal of Biological Chemistry·E Monnet, F Fauvel-Lafève
Oct 19, 2001·European Journal of Biochemistry·B T AtkinsonS P Watson
Jul 12, 2002·The Journal of Biological Chemistry·Thomas M ChiangVirginia Woo-Rasberry
Aug 17, 2002·The Journal of Biological Chemistry·Bernhard NieswandtStefan Offermanns
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·B T AtkinsonS P Watson
Aug 1, 1963·The Journal of Physiology·G V BORN, M J CROSS

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Citations

Nov 12, 2013·Antioxidants & Redox Signaling·Desmond D MurphySarah O'Neill
Jan 31, 2019·The Journal of Biological Chemistry·Eduardo I CardenasRoberto Adachi
Mar 11, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jocelyn M AugerJohan W M Heemskerk
May 4, 2021·Frontiers in Cell and Developmental Biology·Wolfgang M J ObermannJohannes A Eble

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