PMID: 7536280Apr 1, 1995Paper

Differential sensitivity of thoracic malignant tumors to adenovirus-mediated drug sensitization gene therapy

The Journal of Thoracic and Cardiovascular Surgery
W R SmytheL R Kaiser

Abstract

Malignant mesothelioma may prove to be an attractive candidate for somatic gene therapy with replication-deficient recombinant adenovirus transfer of a toxic, or drug sensitization gene. Transfer of the herpes simplex thymidine kinase type I gene (HSVtk), followed by exposure to the acyclic nucleoside drug ganciclovir, has been shown to be an effective tumor cell killing system. To study generalized applicability, we tested a number of thoracic malignant cell lines for their sensitivity to gancyclovir after infection with an adenoviral vector containing the HSVtk gene (Ad.RSVtk). Using the concentration of gancyclovir required to kill 50% of the cells (IC50) as a measure of sensitivity, we detected variable sensitivity among cell lines, with mesothelioma most sensitive (IC50 = 0.075 to 2.8 mumol/L gancyclovir), and non-small-cell carcinoma lines having an intermediate sensitivity (IC50 = 1.5 to 100 mumol/L). In contrast, an ovarian carcinoma line was extremely resistant (IC50 > 2000 mumol/L). To study the possible mechanisms for these differences, we studied cell lines with regard to their ability to be infected with an adenoviral vector containing a marker gene (Ad.CMVlacZ) and expression of the vitronectin receptor alpha v (a...Continue Reading

References

Oct 1, 1992·The American Journal of the Medical Sciences·S Paul, S Dummer
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·N BrünnerJ A Zwiebel
Jul 1, 1988·Reviews of Infectious Diseases·T Matthews, R Boehme
Jun 1, 1994·The Annals of Thoracic Surgery·W R SmytheS M Albelda

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Citations

Dec 21, 2000·Current Oncology Reports·S G Swisher, J A Roth
Feb 1, 1997·Critical Reviews in Oncology/hematology·H C HoogstedenT van Gelder
Jul 30, 2005·Expert Opinion on Biological Therapy·Delia J NelsonRobbert van der Most
Nov 1, 1996·American Journal of Obstetrics and Gynecology·K BehbakhtJ Boyd
Sep 22, 1998·Pharmaceutical Development and Technology·M A CroyleG L Amidon
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·M V Blagosklonny, W S el-Deiry

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