Differential signaling after beta1 integrin ligation is mediated through binding of CRKL to p120(CBL) and p110(HEF1).

The Journal of Biological Chemistry
M SattlerJ D Griffin

Abstract

CRKL is an SH2-SH3-SH3 adapter protein that is a major substrate of the BCR/ABL oncogene. The function of CRKL in normal cells is unknown. In cells transformed by BCR/ABL we have previously shown that CRKL is associated with two focal adhesion proteins, tensin and paxillin, suggesting that CRKL could be involved in integrin signaling. In two hematopoietic cell lines, MO7e and H9, we found that CRKL rapidly associates with tyrosine-phosphorylated proteins after cross-linking of beta1 integrins with fibronectin or anti-beta1 integrin monoclonal antibodies. The major tyrosine-phosphorylated CRKL-binding protein in the megakaryocytic MO7e cells was identified as p120(CBL), the cellular homolog of the v-Cbl oncoprotein. However, in the lymphoid H9 cell line, the major tyrosine-phosphorylated CRKL-binding protein was p110(HEF1). In both cases, this binding was mediated by the CRKL SH2 domain. Interestingly, although both MO7e and H9 cells express p120(CBL) and p110(HEF1), beta1 integrin cross-linking induces tyrosine phosphorylation of p120(CBL) (but not p110(HEF1)) in MO7e cells and of p110(HEF1) (but not p120(CBL)) in H9 cells. In both cell types, CRKL is constitutively complexed to C3G, SOS, and c-ABL through its SH3 domains, and ...Continue Reading

References

Aug 1, 1992·Molecular and Cellular Biology·M MatsudaM Shibuya
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W Y LangdonH C Morse
Dec 8, 1995·The Journal of Biological Chemistry·R SalgiaL B Chen
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S TanakaK Nagashima
Sep 15, 1995·The Journal of Biological Chemistry·R de JongJ Groffen
Apr 14, 1995·Science·E A Clark, J S Brugge
Jul 1, 1995·Molecular and Cellular Biology·H MeisnerM P Czech
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Jul 15, 1996·The Journal of Clinical Investigation·G BazzoniM E Hemler

❮ Previous
Next ❯

Citations

May 10, 2001·International Journal of Hematology·M Sattler, J D Griffin
Nov 26, 2009·Cellular and Molecular Life Sciences : CMLS·Nadezhda TikhmyanovaErica A Golemis
Feb 21, 2003·Current Opinion in Neurobiology·R Jeroen Pasterkamp, Alex L Kolodkin
Feb 17, 2000·Trends in Cell Biology·G M O'NeillE A Golemis
Nov 25, 2005·Stem Cells and Development·Gurudutta U GangenahalliPratibha M Luthra
Jun 24, 2000·Molecular and Cellular Biology·S F LawE A Golemis
Sep 3, 2010·PloS One·Nadezhda TikhmyanovaErica A Golemis
Feb 29, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric F SchmidtStephen M Strittmatter
Sep 25, 2010·Journal of the American Society of Nephrology : JASN·Jianyong XuMiguel A Esteban
Apr 24, 2015·Thrombosis and Haemostasis·Giovanni CimminoPaolo Golino
Mar 21, 2002·Expert Opinion on Therapeutic Targets·T KindlerT Fischer
Jul 24, 2004·Hematology/oncology Clinics of North America·Junia V Melo, Michael W N Deininger
Aug 11, 2017·Antioxidants & Redox Signaling·Amro Elgheznawy, Ingrid Fleming
May 10, 2008·Journal of Cellular Biochemistry·Tetsuya NakamotoSeiichi Kitagawa
May 28, 2010·Journal of Cellular Biochemistry·Angelina FeliciDonald P Bottaro
Aug 27, 2005·The Journal of Biological Chemistry·Qing DengJoseph T Barbieri
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C B Thien, W Y Langdon
Jun 28, 2011·Molecular Endocrinology·Renjini Ambika PadmanabhanMalini Laloraya
Feb 6, 2002·The Journal of Biological Chemistry·Takahiro SuzukiHisamaru Hirai
Nov 3, 1998·Immunology and Cell Biology·C B Thien, W Y Langdon
Dec 23, 1999·The Journal of Biological Chemistry·N Uemura, J D Griffin
Aug 22, 2002·The Journal of Biological Chemistry·Mingzhe Zheng, Paula J McKeown-Longo

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.