Differential signaling of inducible nitric oxide synthase induction in Mycobacterium tuberculosis infected alveolar epithelial cell line A549 in response to cytokines IFN-γ, TNF-α and IL-1β

International Journal of Mycobacteriology
Sugata RoyMridula Bose

Abstract

In earlier studies, it was shown that ex vivo Mycobacterium tuberculosis-infected type II alveolar epithelial cells generate de novo nitric oxide (NO), but the mycobactericidal quantity of NO was released only by stimulation of these cells with proinflammatory cytokines, i.e. IFN-γ, TNF-α and IL-1β. In the present communication, it was demonstrated that M. tuberculosis-infected/mycobacterial antigens stimulated cells utilize both, JAK-STAT and NF-κB pathways for the induction of inducible Nitric Oxide Synthase (iNOS) mRNA and NO production. Alveolar epithelial cell line A549 were either infected with M. tuberculosis or stimulated with M. tuberculosis components. Confocal microscopy, NO estimation and EMSA were performed on the infected/stimulated A549 cells. Nuclear extracts prepared from M. tuberculosis infected A549 cells alone or stimulated with IFN-γ or a combination of three cytokines (IFN-γ, TNF-α and IL-1β) formed DNA protein complexes with probes from both -5.2kb region (specific for binding of STAT-1 protein) and -5.8kb region (specific for binding of both STAT-1 and NF-κB) of the iNOS promoter. However, TNF-α or IL-1β stimulated M. tuberculosis-infected A549 cells showed no protein DNA complexes with construct from -5...Continue Reading

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Citations

Mar 24, 2016·Developmental and Comparative Immunology·Yina ShaoXuemei Duan
Oct 4, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Huafeng Zhang, Bingwei Sun
Jul 4, 2020·Journal of Leukocyte Biology·Tamara Silva RodriguesVânia Luiza Deperon Bonato
Jun 20, 2020·Journal of Leukocyte Biology·Tamara Silva RodriguesVânia Luiza Deperon Bonato

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