Differential staining of ocular goblet cells.

Eye
G G Adams, P N Dilly

Abstract

Millipore filters were used to obtain sheets of cells from the ocular surface. Using Periodic Acid Schiff-haematoxylin the intracellular neutral mucus of the goblet cells stains a brilliant, bright pink and the cell nuclei dark blue making it possible to observe the epithelial cells and the goblet cell population. In certain ocular surface diseases the size of the PAS-haematoxylin staining goblet cell population is reduced, returning towards normal as ocular health improves. These observations had previously been interpreted as showing degeneration followed by regeneration of the goblet cells. However, Alcian blue stains an acidic mucus, which does not stain with PAS-haematoxylin. Acidic mucus containing goblet cells have been revealed using Alcian blue staining when added to the PAS stained cells in conditions that have previously been shown to have reduced goblet cell population when assessed by PAS-haematoxylin staining. This suggests that the staining properties of some of the goblet cells have changed. The extra goblet cells revealed by Alcian Blue staining makes the total of goblet cells present close to normal in numbers. Improved clearing methods and cytological preservation has revealed that there is also a population ...Continue Reading

References

Dec 1, 1977·American Journal of Ophthalmology·P R EgbertD M Maurice
Feb 1, 1976·Biorheology·M LittD P Wolf
Jan 1, 1988·Eye·G G AdamsC M Kirkness
Oct 1, 1980·Archives of Ophthalmology·J V GreinerM R Allansmith

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Citations

Jan 4, 1998·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·A MicaliG Ferreri
Feb 17, 1999·Experimental Eye Research·S J HicksS D Carrington
Dec 20, 2005·Veterinary Ophthalmology·Aline A BolzanJosé L Laus
Jul 27, 1999·Current Eye Research·Y C DieboldJ M Herreras
Aug 1, 1997·Diagnostic Cytopathology·M YağmurS Varinli
Feb 16, 2021·Journal of Lipid Research·Maryam HonarbakhshLoredana Quadro
May 1, 2021·Experimental Eye Research·Mike YangMichelle Senchyna
Jun 4, 2021·Experimental and Molecular Pathology·Laurie B JosephJeffrey D Laskin

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