Differential subcellular distribution and colocalization of the microsomal and soluble epoxide hydrolases in cultured neonatal rat brain cortical astrocytes.

Journal of Neuroscience Research
Seema RawalAmruthesh C Shivachar

Abstract

The microsomal epoxide hydrolase (mEH) and soluble epoxide hydrolase (sEH) enzymes exist in a variety of cells and tissues, including liver, kidney, and testis. However, very little is known about brain epoxide hydrolases. Here we report the expression, localization, and subcellular distribution of mEH and sEH in cultured neonatal rat cortical astrocytes by immunocytochemistry, subcellular fractionation, Western blotting, and radiometric enzyme assays. Our results showed a diffuse immunofluorescence pattern for mEH, which colocalized with the astroglial cytoskeletal marker glial fibrillary acidic protein (GFAP). The GFAP-positive cells also expressed sEH, which was localized mainly in the cytoplasm, especially in and around the nucleus. Western blot analyses revealed a distinct protein band with a molecular mass of approximately 50 kDa, the signal intensity of which increased about 1.5-fold in the microsomal fraction over the whole-cell lysate and other subcellular fractions. The polyclonal anti-human sEH rabbit serum recognized a protein band with a molecular mass similar to that of the affinity-purified sEH protein (approximately 62 kDa), the signal intensity of which increased over 1.7-fold in the 105,000g supernatant fracti...Continue Reading

References

Jan 1, 1991·Brain Research. Brain Research Reviews·A MinnG Siest
Oct 1, 1990·The American Journal of Physiology·E F EllisS C Amruthesh
May 1, 1983·Analytical Biochemistry·S S GillB D Hammock
Jun 1, 1983·Archives of Biochemistry and Biophysics·N ChacosR W Estabrook
Jan 1, 1980·Annual Review of Pharmacology and Toxicology·A Y Lu, G T Miwa
Jan 1, 1995·Drug Metabolism Reviews·V Ravindranath, M R Boyd
Dec 1, 1995·Toxicology Letters·V RavindranathP S Tirumalai
Oct 10, 1995·Analytical Biochemistry·B BorhanB D Hammock
Aug 13, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·A J Draper, B D Hammock
Dec 5, 2002·Archives of Biochemistry and Biophysics·F Peter Guengerich
Jan 23, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shelley Brown DuTeauxMarion G Miller
Mar 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ahmed E EnayetallahDavid F Grant
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Christophe Morisseau, Bruce D Hammock
Jul 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kara R SchmelzerBruce D Hammock
Nov 30, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ahmed E EnayetallahDavid F Grant
Jan 10, 2006·Methods in Enzymology·Michael ArandFranz Oesch
Apr 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ines P KoernerNabil J Alkayed

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Citations

Dec 16, 2011·Journal of Medicinal Chemistry·Hong C Shen, Bruce D Hammock
Jun 24, 2009·Prostaglandins & Other Lipid Mediators·Jeffrey J IliffNabil J Alkayed
Aug 6, 2014·Biochimica Et Biophysica Acta·Arthur A Spector, Hee-Yong Kim
Jan 29, 2019·Frontiers in Pharmacology·Jun-Yan Liu
Sep 4, 2019·The Journal of Clinical Investigation·Jiong HuIngrid Fleming
Nov 20, 2020·Nutrients·Morteza SarparastKin Sing Stephen Lee

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