Differential subcellular expression of protein kinase C betaII in breast cancer: correlation with breast cancer subtypes

Breast Cancer Research and Treatment
Yesim Gökmen-PolarSunil Badve

Abstract

Protein kinase C betaII (PKCβII) represents a novel potential target for anticancer therapies in breast cancer. In order to identify patient subgroups which might benefit from PKC-targeting therapies, we investigated the expression of PKCβII in human breast cancer cell lines and in a tissue microarray (TMA). We first screened breast cancer cell line representatives of breast cancer subtypes for PKCβII expression at the mRNA and at the protein levels. We analyzed a TMA comprising of tumors from 438 patients with a median followup of 15.4 years for PKCβII expression by immunohistochemistry along with other prognostic factors in breast cancer. Among a panel of human breast cancer cell lines, only MDA-MB-436, a triple negative basal cell line, showed overexpression for PKCβII both at the mRNA and at the protein levels. In breast cancer patients, cytoplasmic expression of PKCβII correlated positively with human epidermal growth factor receptor-2 (HER-2; P = 0.01) and Ki-67 (P = 0.016), while nuclear PKCβII correlated positively with estrogen receptor (ER; P = 0.016). The positive correlation of CK5/6 with cytoplasmic PKCβII (P = 0.033) lost statistical significance after adjusting for multiple comparisons (P = 0.198). Cytoplasmic PK...Continue Reading

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Citations

Jul 6, 2010·Breast Cancer Research and Treatment·Yesim Gökmen-PolarKathy D Miller
Dec 18, 2013·Oncogene·R GargM G Kazanietz
May 6, 2014·Frontiers in Oncology·Julie A WallaceMichael C Ostrowski
Jan 23, 2019·EMBO Reports·Yesim Gökmen-PolarSunil S Badve
Aug 1, 2018·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·R K SinghA Acharya

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