Differential Subcellular Localization of Leishmania Alba-Domain Proteins throughout the Parasite Development

PloS One
Aurélien DupéBarbara Papadopoulou

Abstract

Alba-domain proteins are RNA-binding proteins found in archaea and eukaryotes and recently studied in protozoan parasites where they play a role in the regulation of virulence factors and stage-specific proteins. This work describes in silico structural characterization, cellular localization and biochemical analyses of Alba-domain proteins in Leishmania infantum. We show that in contrast to other protozoa, Leishmania have two Alba-domain proteins, LiAlba1 and LiAlba3, representative of the Rpp20- and the Rpp25-like eukaryotic subfamilies, respectively, which share several sequence and structural similarities but also important differences with orthologs in other protozoa, especially in sequences targeted for post-translational modifications. LiAlba1 and LiAlba3 proteins form a complex interacting with other RNA-binding proteins, ribosomal subunits, and translation factors as supported by co-immunoprecipitation and sucrose gradient sedimentation analysis. A higher co-sedimentation of Alba proteins with ribosomal subunits was seen upon conditions of decreased translation, suggesting a role of these proteins in translational repression. The Leishmania Alba-domain proteins display differential cellular localization throughout the ...Continue Reading

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Citations

Feb 24, 2016·Biochimica Et Biophysica Acta·Manish GoyalUday Bandyopadhyay
Oct 14, 2016·Cell Death & Disease·Prasad Kottayil PadmanabhanBarbara Papadopoulou
Dec 18, 2016·Molecular and Biochemical Parasitology·Leticia Pérez-DíazSantuza M R Teixeira
Aug 28, 2018·RNA Biology·Helisa Helena WippelLysangela Ronalte Alves
May 5, 2020·Nucleic Acids Research·Tiago R FerreiraPegine B Walrad
Aug 6, 2020·Scientific Reports·Bruno Guedes AguiarBarbara Papadopoulou
Apr 29, 2020·International Journal of Molecular Sciences·Zemfira N KaramyshevaAndrey L Karamyshev
Dec 10, 2020·Journal of Proteomics·Paola A NocuaConcepción J Puerta
Feb 11, 2021·International Journal of Molecular Sciences·Alena NáprstkováDavid Honys
Jun 10, 2021·Journal of Structural Biology·Jie GaoXiaoming Tu

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Methods Mentioned

BETA
Acetylation
transfection
PCR
co-immunoprecipitation
co-IP
possible
immunoprecipitation
protein folding
pull-downs
flow cytometry

Software Mentioned

Phyre
MEGA5
ProteinPilot
Scaffold
GPEET
Mascot
ImagePro Plus
BioEdit
ImageJ
Analyst

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