PMID: 1212196Sep 1, 1975Paper

Differential subnuclear distribution of polyadenylate-rich ribonuclei acid during induction of egg-yolk protein synthesis in male Xenopus liver by oestradiol-17 beta

The Biochemical Journal
J R Tata, B Baker

Abstract

A 4-8-fold increase in the rate of hepatic nuclear RNA synthesis occurred within 11 h after a single injection of oestradiol-17 beta to male Xenopus to induce egg-yolk protein synthesis. 2. By using a gentle procedure for fractionating nuclei into their major structurally different components [J. R. Tata& B. Baker (1974) Exp. Cell Res. 83. 111-124], it was found that the hormone-induced increase in the total amount of newly made RNA was associated with a 2-10-fold increase in the poly(A) content of nuclear RNA. 3. When the poly (A) content of nuclear RNA was determined by hybridization to poly[3H](U) or specific binding to oligo(dT)-cellulose, most of the increase (10-fold) in poly (A) content of newly synthesized RNA was associated with the euchromatin fractions, whereas the increase was less marked in the other subnuclear fractions. 4. Resolution of nuclear RNA into poly (A)-poor and poly(A)-rich RNA species by chromatography on oligo(dT)-cellulose, followed by polyacrylamide-gel electrophoresis with sodium dodecyl sulphate or in the pressence of 99% formamide, revealed that the hormone caused a preferential enhancement of high-molecular-weight (25S-60S) poly (A)-rich HnRNA (heterogeneous nuclear RNA,) much of which was assoc...Continue Reading

Citations

Feb 11, 1982·Biochemical and Biophysical Research Communications·B Schulz-Harder, J R Tata
Sep 1, 1976·Cell·J R Tata
Mar 24, 1980·FEBS Letters·Z A Medvedev, M N Medvedeva
Feb 27, 2001·Molecular and Cellular Endocrinology·D MartínA S Raikhel
Jul 1, 1979·Journal of Steroid Biochemistry·J R Tata
Feb 14, 1979·Biochemical and Biophysical Research Communications·J M Grouse, L D Grouse
May 1, 1991·General and Comparative Endocrinology·E H LimT J Lam

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