Differential susceptibility of fish and rat liver cells to oxidative stress and cytotoxicity upon exposure to prooxidants

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Michelle A RauRichard T Di Giulio

Abstract

Species differences in the ability to cope with pollutant-mediated oxidative stress can provide insight into the mechanisms behind both the mode of toxicity of a specific chemical as well as the different ways in which an organism may deal with such stressors. In this study, the effects of exposure to model prooxidants on parameters of oxidative stress were investigated in liver cells from both fish (PLHC-1) and rat (H4IIE). The goals of this study were to compare the oxidative stress response of these cell lines and to assess the relative utility of several different measures of oxidative stress as signals preceding cytotoxicity. Cellular response to two model prooxidants, copper and Fenton reagents (ferrous sulfate plus hydrogen peroxide), was assessed by measuring cytotoxicity, lipid peroxidation, total glutathione (GSHT), and percent glutathione disulfide (%GSSG). Additionally, transcriptional activation of an antioxidant response element (ARE) reporter gene was measured using the chloramphenicol acetyltransferase (CAT) assay in response to these chemicals. In general, the fish cells were more sensitive than rat cells to prooxidants, and the assays for lipid peroxidation and ARE reporter gene activation were more sensitive ...Continue Reading

References

Jan 1, 1992·Journal of Biochemical Toxicology·Y SinghM G Miller
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
May 1, 1994·Toxicology and Applied Pharmacology·N SugiharaK Kohashi
Jun 7, 1996·Journal of Toxicology and Environmental Health·K FuronoN Sugihara
Jan 15, 1997·Archives of Biochemistry and Biophysics·J O OssolaM L Tomaro
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W Wasserman, W E Fahl
Jun 25, 1998·Environmental Health Perspectives·K A KellyE D Levin
Oct 26, 1999·The Journal of Experimental Zoology·P VendittiS Di Meo
May 18, 2000·Toxicology·F ShokriM Ghazi-Khansari
Jan 13, 2001·Chemico-biological Interactions·D J Long, A K Jaiswal
Mar 20, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Z VarankaM Abrahám
Nov 1, 2002·Marine Environmental Research·Robert T PhamEvan P Gallagher
Feb 6, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Chen-Huei HuangWenlung Chen
Aug 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K MertensV Rogiers

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Citations

May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Lee Macomber, James A Imlay
Dec 26, 2006·Journal of Bacteriology·Lee MacomberJames A Imlay
Jun 21, 2014·Toxicon : Official Journal of the International Society on Toxinology·Constanze PietschPatricia Burkhardt-Holm
Mar 26, 2013·The Science of the Total Environment·Dolores FernándezMar Babín
Feb 2, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E FarmenK E Tollefsen
May 16, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A JosH Segner
Jul 22, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Luka TravenVladimir Mićović
Jul 8, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E F FinneK E Tollefsen
Dec 8, 2007·Aquatic Toxicology·Stephanie K BoppKatja Knauer
Jan 21, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Volodymyr I Lushchak, Tetyana V Bagnyukova
Jan 24, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Volodymyr I Lushchak, Tetyana V Bagnyukova
Nov 30, 2014·Bulletin of Environmental Contamination and Toxicology·Mazyar YazdaniKetil Hylland
Jul 12, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Juan SunJunichi Misumi
Jun 28, 2005·Ecotoxicology and Environmental Safety·V V Arkhipchuk, N N Garanko
Jun 17, 2015·Chemico-biological Interactions·Andreea Iren SerbanAnca Dinischiotu
Jul 11, 2014·Redox Biology·Toshiki NakanoMinoru Sato
Jun 18, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chin-Shiu HuangLan-Chi Chiu
Jun 8, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Della TorreI Corsi
Jun 28, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Halina I FalfushynskaYoon Kwon Nam
Jun 29, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Constanze Pietsch, Ranka Junge
Oct 11, 2016·Ecotoxicology and Environmental Safety·Leobardo Manuel Gómez-OlivánRicardo Pérez-Pastén Borja
Oct 28, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David F J LisserMichael P Wilkie
Jun 11, 2019·Frontiers in Physiology·Lais Cavalieri ParedesTarcio Teodoro Braga
Dec 30, 2011·Journal of Inorganic Biochemistry·Jeffrey T Rubino, Katherine J Franz

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