Differential targeting of T- and N-cadherin in polarized epithelial cells.

The Journal of Biological Chemistry
E Koller, Barbara Ranscht

Abstract

To test whether glycosyl phosphatidylinositol-linked T-cadherin is a component of cell junctions like classical cadherins, we have examined its distribution and targeting in polarized epithelial cells. In vivo, T-cadherin was detected on the apical cell surface of the chick intestinal epithelium. In cultures of transfected Madin-Darby canine kidney cells, T-cadherin was also expressed apically, whereas classical N-cadherin resided basolaterally. Both cadherins were directly targeted to their respective membrane domains. Mutant proteins were expressed in Madin-Darby canine kidney cells to identify the regions responsible for differential cadherin localization. NDeltacyt, an N-cadherin cytoplasmic domain deletion mutant, was stably distributed basolaterally. This mutant was transported to both the apical and basolateral membrane compartments, followed by preferential removal from the apical surface. T-NDeltacyt, a T-cadherin mutant with the N-cadherin cytoplasmic domain deletion, was localized basolaterally, whereas N-TGPI, a GPI-anchored N-cadherin mutant, resided at the apical domain. The T-cadherin carboxyl-terminal 76 amino acids contain the apical targeting signal and include the signal for GPI anchor attachment. Basolateral...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 6, 1991·Cell·C R Hopkins
Jan 1, 1990·Annual Review of Cell Biology·G A Cross
Jan 1, 1989·Methods in Cell Biology·E Rodriguez-BoulanL Graeve
Jan 1, 1985·The Journal of Membrane Biology·L Gonzalez-MariscalM Cereijido
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·J NakayamaM Fukuda
Feb 1, 1995·Current Opinion in Genetics & Development·C Kirkpatrick, M Peifer
Aug 1, 1994·Current Opinion in Cell Biology·K Matter, I Mellman
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Jan 1, 1993·Annual Review of Cell Biology·I S TrowbridgeC R Hopkins
Jun 1, 1993·Seminars in Cell Biology·A NagafuchiM Takeichi

❮ Previous
Next ❯

Citations

Sep 1, 2005·Virchows Archiv : an International Journal of Pathology·Joachim ArnemannWiltrud Coerdt
Jan 20, 2004·Experimental Cell Research·Danila IvanovThérèse Resink
Nov 5, 1997·Biochimica Et Biophysica Acta·O NosjeanB Roux
Mar 2, 2010·Nature Structural & Molecular Biology·Carlo CiattoLawrence Shapiro
Jan 2, 2003·Molecular and Cellular Biology·Zhi-yong HuangDavid H Gutmann
Dec 30, 2011·American Journal of Nephrology·Adam T Whaley-ConnellJames R Sowers
Oct 12, 2000·Biochemical and Biophysical Research Communications·T NiermannT Resink
Jun 13, 2002·The Journal of Investigative Dermatology·Shuxia ZhouYoshiki Miyachi
Mar 2, 2005·Biochemical and Biophysical Research Communications·Farida GoubaevaJay Yang
Feb 24, 2007·Developmental Biology·Amalia RosnerBaruch Rinkevich
Jan 25, 2017·Pulmonary Pharmacology & Therapeutics·Andrea BiancoAurora Daniele
Feb 11, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria PhilippovaThérèse Resink
Aug 16, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manjunath B JoshiThérèse J Resink
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Michael GoldbergDorothy Warburton
Jul 15, 2005·Molecular and Cellular Biochemistry·K RubinaV Tkachuk
Apr 20, 2013·Oncology Letters·Xin-Suo DuanLi-Xin Sun
Apr 29, 2009·Cellular Signalling·Maria PhilippovaTherese J Resink

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.