Differential thiol oxidation of the signaling proteins Akt, PTEN or PP2A determines whether Akt phosphorylation is enhanced or inhibited by oxidative stress in C2C12 myotubes derived from skeletal muscle

The International Journal of Biochemistry & Cell Biology
Pearl Lin TanPeter G Arthur

Abstract

Oxidative stress, caused by excess reactive oxygen species (ROS), has been hypothesized to cause or exacerbate skeletal muscle wasting in a number of diseases and chronic conditions. ROS, such as hydrogen peroxide, have the potential to affect signal transduction pathways such as the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3 K)/Akt pathway that regulates protein synthesis. Previous studies have found contradictory outcomes for the effect of ROS on the PI3K/Akt signaling pathway, where oxidative stress can either enhance or inhibit Akt phosphorylation. The apparent contradictions could reflect differences in experimental cell types or types of ROS treatments. We replicate both effects in myotubes of cultured skeletal muscle C2C12 cells, and show that increased oxidative stress can either inhibit or enhance Akt phosphorylation. This differential response could be explained: thiol oxidation of Akt, but not the phosphatases PTEN or PP2A, caused a decline in Akt phosphorylation; whereas the thiol oxidation of Akt, PTEN and PP2A increased Akt phosphorylation. These observations indicate that a more complete understanding of the effects of oxidative stress on a signal transduction pathway comes not only from identifying the ...Continue Reading

References

Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Nov 10, 2001·Trends in Biochemical Sciences·D P Brazil, B A Hemmings
Feb 23, 2002·Cellular Signalling·Nick R Leslie, C Peter Downes
Mar 26, 2002·Cancer Letters·Maria Cristina C Gomes-Marcondes, Michael J Tisdale
Mar 28, 2002·The Journal of Biological Chemistry·Seung-Rock LeeSue Goo Rhee
Jun 11, 2002·Biochemical and Biophysical Research Communications·R K Rao, L W Clayton
Jul 31, 2002·Archives of Biochemistry and Biophysics·Debbie SommerPaul M Stemmer
Jan 9, 2003·Structure·Xin HuangXiaotian Zhu
Apr 12, 2003·FEBS Letters·Barry Halliwell
Jul 16, 2003·Cold Spring Harbor Symposia on Quantitative Biology·N WinnN Rosenthal
Aug 30, 2003·Trends in Cell Biology·Maria Luisa Sulis, Ramon Parsons
Oct 3, 2003·The Journal of Biological Chemistry·Hiroaki MurataTakahito Kondo
Oct 9, 2003·The EMBO Journal·Nick R LeslieC Peter Downes
Jan 28, 2004·Cellular and Molecular Life Sciences : CMLS·P ChelikaniP C Loewen
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Robert W Jackman, Susan C Kandarian
Nov 27, 2004·Journal of Cell Science·Rosa Ana LacalleSantos Mañes
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Aug 10, 2005·The International Journal of Biochemistry & Cell Biology·David J Glass
Aug 30, 2005·The International Journal of Biochemistry & Cell Biology·Nathaniel J Szewczyk, Lewis A Jacobson
Jan 7, 2006·The Journal of Physiology·Russell S RichardsonPierre G Carlier
Jan 28, 2006·Assay and Drug Development Technologies·Hubert Th Wolterbeek, Astrid J G M van der Meer
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Cullen M TaniguchiC Ronald Kahn
Sep 22, 2006·Antioxidants & Redox Signaling·Nick R Leslie
Sep 22, 2006·Antioxidants & Redox Signaling·Cristen PantanoYvonne M W Janssen-Heininger
Sep 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Thea Shavlakadze, Miranda Grounds
Jan 30, 2007·Journal of Experimental Botany·Chuan-Ming YehHao-Jen Huang
Feb 3, 2007·Muscle & Nerve·Jennifer S Moylan, Michael B Reid
Apr 12, 2008·Cell Death and Differentiation·A M MackeyT G Cotter
Jun 11, 2008·Current Opinion in Clinical Nutrition and Metabolic Care·Peter G ArthurThea Shavlakadze
Jun 12, 2008·Free Radical Biology & Medicine·Christine C Winterbourn, Mark B Hampton
Mar 13, 2009·Biochimie·Mahdieh SadidiEva L Feldman
Sep 26, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·T LangT B Harris
Feb 25, 2010·Journal of Cell Science·Thea ShavlakadzeMiranda D Grounds
May 1, 2010·The Journal of Biological Chemistry·Alina BerdichevskyAvirup Bose
Jul 20, 2010·The European Journal of Neuroscience·Sorcha FinneganThomas G Cotter
Dec 17, 2011·Journal of Signal Transduction·Elena Barbieri, Piero Sestili
Dec 27, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lalitha DurgadossVijayalakshmi Ravindranath

❮ Previous
Next ❯

Citations

Feb 26, 2016·Free Radical Biology & Medicine·Hiroyuki YamauchiYasuchika Takeishi
Feb 26, 2016·Free Radical Biology & Medicine·Scott K PowersAshley J Smuder
Mar 26, 2016·Molecular Aspects of Medicine·Giuseppe MaulucciShlomo Sasson
May 18, 2016·Free Radical Biology & Medicine·George G RodneyReem Abo-Zahrah
May 26, 2017·Critical Care Medicine·Flavia StanaSabah N A Hussain
Aug 19, 2017·Antioxidants & Redox Signaling·Mauricio Olguín-Albuerne, Julio Morán
Aug 14, 2019·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Kavitha Mukund, Shankar Subramaniam
Feb 11, 2020·Antioxidants & Redox Signaling·Fabio PennaPaola Costelli
Jun 8, 2017·Antioxidants & Redox Signaling·Sue Goo RheeDongmin Kang
May 23, 2018·Oxidative Medicine and Cellular Longevity·Johanna ÁbrigoClaudio Cabello-Verrugio
May 23, 2017·Frontiers in Endocrinology·Lewan ParkerItamar Levinger
Oct 23, 2019·Toxicological Research·Yoshito KumagaiTakamitsu Unoki
Dec 2, 2020·Cells·Giorgio AquilaRosanna Piccirillo
Feb 9, 2021·Experimental Biology and Medicine·Chenyuan LiShengrong Sun
May 7, 2020·Redox Biology·M C Gomez-CabreraJ Viña
Aug 28, 2021·International Journal of Molecular Sciences·Nicholas A ForemanLi Li Ji
Sep 17, 2018·Free Radical Biology & Medicine·Alvaro Martinez GuimeraCarole J Proctor

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cachexia & Brown Fat

Cachexia is a condition associated with progressive weight loss due to severe illness. In cancer patients, it is proposed to occur as a result of tumor-induced energy wasting. Several proteins have been implicated in browning and depletion of white adipose tissue. Here is the latest research on cachexia and brown fat.

Cardiac Cachexia

Cardiac cachexia is a syndrome associated with the progressive loss of muscle and fat mass. It most commonly affects patients with heart failure and can significantly decrease the quality of life and survival in these patients. Here is the latest research on cardiac cachexia.