Differential time-course of induction of rat liver gamma-glutamyltransferase and drug-metabolizing enzymes in the endoplasmic reticulum, Golgi and plasma membranes after a single phenobarbital injection. Evaluation of protein variations by two-dimensional electrophoresis

Cell Biochemistry and Function
B AntoineM M Galteau

Abstract

This study was conducted to follow as a function of time the activity of gamma-glutamyltransferase in the various membranes of rat liver cells after a single dose of phenobarbital (PB) (75 mg kg-1 body weight). Gamma-glutamyltransferase induction was maximal 24 h after PB treatment in both the rough endoplasmic reticulum and the plasma membranes. This pattern of induction differed from that of some drug metabolizing enzymes. While total cytochrome P-450 content was enhanced mainly in endoplasmic reticulum until 48 h after PB treatment, UDP-glucuronosyltransferase activity was not greatly altered by PB under the same conditions. The comparison of two-dimensional electrophoretic polypeptide profiles of each subcellular membrane isolated from control and phenobarbital-treated rats revealed important variations induced by PB. In plasma membranes, the heaviest subunit (apparent Mr = 60 x 10(3)) of hepatic gamma-glutamyltransferase was provisionally identified as a collection of polypeptide which differ only by their pI. The concentration of these polypeptides was smaller in the endoplasmic reticulum where they were of lower apparent molecular mass. This suggests that the gamma-glutamyltransferase precursor is already processed at th...Continue Reading

References

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Citations

Sep 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·B AntoineG Siest
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elisabetta GianazzaIngrid Miller
Jan 1, 1989·Drug Metabolism Reviews·G SiestD Bagrel
Mar 5, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shengmin SangChung S Yang
Jun 1, 1996·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·R P Donn, W E Ollier
Jun 12, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Keisuke NagatsumaTomokazu Matsuura

Related Concepts

Plasma Membrane
Cytochrome P-450 Oxygenase
SDS-PAGE
Endoplasmic Reticulum
Enzyme Induction
Gamma-glutamyl transferase
Golgi Apparatus
Liver
Mitochondria, Liver
Luminal

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