Differential time-course of induction of rat liver gamma-glutamyltransferase and drug-metabolizing enzymes in the endoplasmic reticulum, Golgi and plasma membranes after a single phenobarbital injection. Evaluation of protein variations by two-dimensional electrophoresis

Cell Biochemistry and Function
B AntoineM M Galteau


This study was conducted to follow as a function of time the activity of gamma-glutamyltransferase in the various membranes of rat liver cells after a single dose of phenobarbital (PB) (75 mg kg-1 body weight). Gamma-glutamyltransferase induction was maximal 24 h after PB treatment in both the rough endoplasmic reticulum and the plasma membranes. This pattern of induction differed from that of some drug metabolizing enzymes. While total cytochrome P-450 content was enhanced mainly in endoplasmic reticulum until 48 h after PB treatment, UDP-glucuronosyltransferase activity was not greatly altered by PB under the same conditions. The comparison of two-dimensional electrophoretic polypeptide profiles of each subcellular membrane isolated from control and phenobarbital-treated rats revealed important variations induced by PB. In plasma membranes, the heaviest subunit (apparent Mr = 60 x 10(3)) of hepatic gamma-glutamyltransferase was provisionally identified as a collection of polypeptide which differ only by their pI. The concentration of these polypeptides was smaller in the endoplasmic reticulum where they were of lower apparent molecular mass. This suggests that the gamma-glutamyltransferase precursor is already processed at th...Continue Reading


Nov 1, 1978·The Journal of Cell Biology·K E HowellG E Palade
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L Anderson, N G Anderson
Sep 16, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E ScherberichG Pfleiderer
Jan 1, 1972·Preparative Biochemistry·D J MorréS E Nyquist
Nov 8, 1965·Biochimica Et Biophysica Acta·A Fleck, D Begg
Jun 27, 1983·FEBS Letters·M A Capraro, R P Hughey
Sep 1, 1983·Journal of Biochemistry·Y MatsudaN Katunuma
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Jul 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B AntoineG Siest
Jul 30, 1981·Biochemical and Biophysical Research Communications·E Le ProvostJ P Leroux
Mar 1, 1982·Archives of Biochemistry and Biophysics·G P Vlasuk, F G Walz
Sep 2, 1980·Biochimica Et Biophysica Acta·M A Kaderbhai, R B Freedman


Sep 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·B AntoineG Siest
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elisabetta GianazzaIngrid Miller
Jan 1, 1989·Drug Metabolism Reviews·G SiestD Bagrel
Mar 5, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shengmin SangChung S Yang
Jun 1, 1996·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·R P Donn, W E Ollier
Jun 12, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Keisuke NagatsumaTomokazu Matsuura

Related Concepts

Plasma Membrane
Cytochrome P-450 Oxygenase
Endoplasmic Reticulum
Enzyme Induction
Gamma-glutamyl transferase
Golgi Apparatus
Mitochondria, Liver

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.