Differential toll-like receptor expression after ex vivo lipopolysaccharide exposure in patients with sepsis and following surgical stress

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Hironori TsujimotoHidetaka Mochizuki

Abstract

Monocytes from septic patients have a reduced capacity to respond to lipopolysaccharide (LPS). We examined whether the same response occurred after surgical injury, and whether this reduced activity was associated with differential monocyte toll-like receptor (TLR) expression. Peripheral blood mononuclear cells (PBMCs) were isolated from septic patients, patients undergoing surgery, and healthy volunteers. Cells were stimulated ex vivo with LPS (1 microg/ml) and stained for CD14, CD16, TLR-2, TLR-4, and HLA-DR surface expression. TLR-2 and -4 expressions were significantly increased in monocytes from both septic and surgical patients. While ex vivo LPS-stimulation significantly increased TNFalpha and IL-1beta production in PBMCs from surgical patients, LPS-stimulation decreased IL-1beta production from septic patients as compared to surgical and control patients. Ex vivo LPS-stimulation induced TLR-4 upregulation in monocytes from both surgical and control patients, but not from septic patients. HLA-DR expression in CD14+CD16+ monocytes was reduced only in septic patients. PBMCs from septic patients, but not following surgical injury, have a reduced capacity to respond to a secondary inflammatory signal, but this defect is not ...Continue Reading

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Dec 23, 2004·Shock·Hironori TsujimotoHidetaka Mochizuki

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Citations

Jun 12, 2008·Langenbeck's Archives of Surgery·Klaus ButtenschoenDaniela Carli Buttenschoen
Sep 8, 2011·Surgical Endoscopy·Konstantinos E TsimogiannisGeorge Basdanis
Aug 27, 2010·Surgery Today·Fumio KimuraMasaru Miyazaki
Apr 24, 2009·Journal of Clinical Immunology·Philip A EfronAlan W Hemming
Feb 17, 2007·Clinical and Experimental Immunology·V FaivreA Haziot
Jun 13, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Daniela RozkovaRadek Spisek
Dec 27, 2013·Mediators of Inflammation·Letizia MezzasomaVincenzo Nicola Talesa
Jun 5, 2016·Critical Care : the Official Journal of the Critical Care Forum·Alexander AsmussenSebastian Grundmann
Dec 28, 2007·Journal of Leukocyte Biology·Steven C GribarDavid J Hackam
Apr 9, 2010·Current Opinion in Infectious Diseases·Klaus ButtenschoenDaniela Carli Buttenschoen
Jun 9, 2016·Annals of Surgery·William AlazawiSatyajit Bhattacharya
Aug 28, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Justyna AgierMagdalena Wiktorska
Feb 19, 2008·Shock·Hironori TsujimotoHidetaka Mochizuki
Nov 8, 2012·Annals of the Royal College of Surgeons of England·F AusaniaB C Jaques
Jun 29, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael T BaileyJohn F Sheridan
Feb 26, 2021·Journal of Immunology Research·Yaroslav V RadzyukevichIsabella R Prokhorenko

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