Differential transcription in defined parts of the insect brain: comparative study utilizing Drosophila melanogaster and Schistocerca gregaria

Invertebrate Neuroscience : in
Thomas RoederOliver Franz

Abstract

The brain of all higher organisms has a modular architecture. Processing of various tasks, such as learning, olfaction, or motor control is performed in specialized brain areas, characterized by morphological and molecular peculiarities. To identify those genes that are transcribed in only one region of the insect brain, we chose two different approaches, differential display PCR and DNA array hybridization, with two different insect species, the desert locust Schistocerca gregaria and the fruitfly Drosophila melanogaster. The optic lobes (centers of visual information processing), the midbrain (the region of the brain where almost all "higher" centers are localized), and the thoracic ganglia (regions required to control various peripheral organs) were compared in both types of experiments. Both, the differential display PCR screen of the different parts of the locust brain as well as the DNA array screen of the Drosophila brain revealed almost identical numbers of transcripts exclusively present in either of the three above-mentioned brain areas. Interestingly, the brain areas with the largest number of differential transcripts are the thoracic ganglia and not the midbrain.

References

Sep 1, 1996·Journal of Neurobiology·P L HanR L Davis
Jul 3, 1998·Nucleic Acids Research·T Roeder
May 15, 1999·Nucleic Acids Research·O FranzT Roeder
Mar 25, 2000·Science·M D AdamsJ C Venter
May 24, 2000·European Journal of Pharmacology·J DegenT Roeder
Nov 10, 2000·Nucleic Acids Research·G SchrammT Roeder
Oct 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christina M GrozingerGene E Robinson

❮ Previous
Next ❯

Citations

Oct 26, 2012·Proceedings. Biological Sciences·Yibayiri O SanogoAlison M Bell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.