Differential transcriptional effects of PTH and estrogen during anabolic bone formation

Journal of Cellular Biochemistry
D von StechowJoseph M Alexander

Abstract

The aim of this study was to compare transcriptional regulation in vivo during anabolic bone formation induced by either estradiol (E2) treatment or intermittent parathyroid hormone[1-34] (PTH) therapy. We utilized an ovariectomized (OVX) mouse model of osteoporosis and transcriptional profiling to identify genes upregulated by either high-dose E2 or PTH. Five weeks post-OVX, the mice were administered either E2 and/or PTH, or vehicle for 4 weeks. Femoral bones were analyzed by microCT and histomorphometry to confirm the anabolic effect of each treatment. OVX vehicle-treated control mice lost metaphyseal trabecular bone, with significant decrease in trabecular number, thickness, and connectivity. Both E2 and PTH treatments increased trabecular and cortical bone indices above the level of the sham operated controls, fully restoring both bone volume and bone mineral density (BMD). Moreover, PTH/E2 combination treatment led to significantly greater increase in cancellous bone and BMD than would be expected from the additive effects of the separate treatments. To determine whether PTH and E2 treatments were stimulating similar bone anabolic mechanisms, or were activating distinct signaling pathways, we compared patterns of gene exp...Continue Reading

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Apr 20, 2006·Journal of Bone and Mineral Metabolism·Chie WadaToshihiko Nagata
Oct 3, 2015·Molecular and Cellular Endocrinology·Freya F JayReinhold G Erben
Apr 26, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alice Fiona Ford-HutchinsonFrank Robert Jirik
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Sep 26, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yong-Jun LiuHong-Wen Deng
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Sep 22, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Zhiguo ZhangDahong Ju
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Oct 26, 2012·Menopause : the Journal of the North American Menopause Society·Zhi-Guo ZhangDa-Hong Ju

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