PMID: 8604295Feb 15, 1996Paper

Differential transcriptional regulation of the apoAI gene by retinoic acid receptor homo- and heterodimers in yeast

Nucleic Acids Research
A J SalernoP Mak

Abstract

Several members of the nuclear receptor superfamily including RXR (retinoid X receptor) bind to a specific retinoic acid response element (site A) of the apoAI promoter. However, transcriptional activation of the apoAI gene by different homo- and heterodimeric forms of RXR or RAR (retinoic acid receptors) cannot be evaluated in mammalian cells, which contain endogenous RXR or RAR. In order to circumvent this limitation, we assessed the DNA-binding activities and transcriptional activation of different homo- and heterodimers of these receptors in yeast. Electrophoretic mobility shift assays (EMSA) demonstrated that yeast expressed RARalpha does not bind to site A of the apoAl promoter, whereas binding of RARbeta to site A is ligand-dependent. Both RARalpha and RARbeta form heterodimers with RXRalpha and bind to site A with high affinity. These DNA-binding studies correlate with the transcriptional data, which indicated that RARbeta but not RARalpha activates transcription from site A in response equally well to 9-cis and all-trans retinoic acids. 9-cis RA is a more potent ligand than all-trans RA to activate transcription via RXR/RAR heterodimers. We conclude that this yeast expression system is a useful tool to elucidate the 't...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A M ChristiansonF C Kafatos
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Aug 1, 1992·Molecular and Cellular Biology·R L WidomS K Karathanasis
Dec 18, 1992·Science·J M LehmannM Pfahl
Feb 10, 1989·Cell·M Beato
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Dec 25, 1994·Nucleic Acids Research·H A FuernkranzP Mak
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D M HeeryR Losson
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B L HallM L Privalsky

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Citations

Jul 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·F Dela Cruz, P Mak
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuet-Kin LeungShuk-Mei Ho
Nov 30, 2006·Neoplasia : an International Journal for Oncology Research·Paul MakShuk-Mei Ho
Oct 21, 2005·Biochimica Et Biophysica Acta·Kent WehmeierArshag D Mooradian
Jul 8, 2008·Biochemical and Biophysical Research Communications·Paul MakK C Balaji

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