Differential Transcriptome Analysis of Early Postnatal Developing Longissimus Dorsi Muscle from Two Pig Breeds Characterized in Divergent Myofiber Traits and Fatness

Animal Biotechnology
Xiaoxing XuRifu Xu

Abstract

Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal muscle fiber characteristics in the longissimus dorsi muscle (LDM) of the Northeast Min pig (NM) and the Changbaishan wild boar (CW) with their body weight approaching 90 kg. The significant divergences in the skeletal muscle fiber phenotypes and fatness traits between the two porcine breeds established an ideal model system for further identifying potential key functional genes that dominated MQTs. Further, a transcriptome profile analysis was performed using the Illumina sequencing method in early postnatal developing LDM from the two breeds at the ages of 42 days. Comparative analysis between these two cDNA libraries showed that there were 17,653 and 22,049 unambiguous tag-mapped sense transcripts detected from NM and CW, respectively. 4522 differentially expressed genes (DEGs) were revealed between the two tissue samples, of them, 4176 genes were found as having been upregulated and 346 genes were identified as ...Continue Reading

References

Sep 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M H Brooke, K K Kaiser
Aug 1, 1993·Bioscience Reports·C RehfeldtK Ender
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A Rudnicki, R Jaenisch
Jun 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Z Zhu, J B Miller
Oct 23, 1997·Genome Research·S Audic, J M Claverie
Feb 18, 1999·Molecular and Cellular Biology·F J KingT M Roberts
Oct 7, 2000·European Journal of Endocrinology·M WautersL F Van Gaal
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Jan 18, 2002·Nature·Yasuhiko MinokoshiBarbara B Kahn
Apr 2, 2004·Nature Medicine·Ronald M EvansYong-Xu Wang
Aug 18, 2004·Journal of Animal Science·L LefaucheurC Le Callennec
Jul 16, 2005·Cell Death and Differentiation·D J KimJ M Peters
Sep 6, 2007·Genome Research·Sorin DraghiciRoberto Romero
Dec 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bert KadereitDavid L Silver
Feb 2, 2008·Science in China. Series C, Life Sciences·HongMei HuYing Wu
Feb 16, 2008·International Review of Cytology·James M Ervasti, Kevin J Sonnemann
Jan 1, 1986·Meat Science·K O HonikelP Roncales
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·David A GrossDavid L Silver
Apr 26, 2012·International Journal of Biological Sciences·Weizhen LuoLongchao Zhang
Feb 13, 2014·The Journal of Biological Chemistry·Diego A MirandaDavid L Silver
Apr 3, 2014·PPAR Research·Lihong Chen, Guangrui Yang

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Citations

Jul 19, 2020·Animals : an Open Access Journal From MDPI·Guofang WuChen Yang
Sep 7, 2021·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Aleš KnollTomáš Urban

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Methods Mentioned

BETA
reverse transcription-PCR
PCR
Solexa sequencing
electrophoresis

Software Mentioned

SPSS12
Solexa

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