Differential trophic effects of basic fibroblast growth factor, insulin-like growth factor-1, and neurotrophin-3 on striatal neurons in culture

Experimental Neurology
N NakaoP Brundin

Abstract

We have examined the trophic effects of basic fibroblast growth factor (bFGF), truncated insulin-like growth factor-1 (tIGF), and neurotrophin-3 (NT-3) on embryonic striatal neurons grown under serum-free culture conditions. Striatal neurons were identified using immunocytochemistry for dopamine- and cyclic AMP-regulated phosphoprotein (DARPP-32). In our serum-free striatal cultures, the survival and the development of DARPP-32- containing neurons were dependent on initial plating density: relatively high density cultures yielded disproportionately increased number of harvested DARPP-32- positive neurons. All three growth factors, bFGF (10 ng/ml), tIGF-1 (50 ng/ml), and NT-3 (50 ng/ml), promoted the survival of DARPP-32-positive neurons, with bFGF being significantly more effective than tIGF-1 and NT-3. Exposure to bFGF also significantly increased the total cell number compared to control cultures, whereas there was only a tendency toward more (20-30%) surviving cells in cultures treated with either tIGF-1 or NT-3. With the concentrations used, only bFGF gave rise to a significant increase (80%) in the number of glial fibrillary acidic protein-immunopositive glia as compared to controls. The most pronounced effect on morpholog...Continue Reading

Citations

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