Differential tumor biological role of the tumor suppressor KAI1 and its splice variant in human breast cancer cells

Oncotarget
Julia MillerUte Reuning

Abstract

The tetraspanin and tumor suppressor KAI1 is downregulated or lost in many cancers which correlates with poor prognosis. KAI1 acts via physical/functional crosstalk with other membrane receptors. Also, a splice variant of KAI1 (KAI1-SP) has been identified indicative of poor prognosis. We here characterized differential effects of the two KAI1 variants on tumor biological events involving integrin (αvß3) and/or epidermal growth factor receptor (EGF-R). In MDA-MB-231 and -435 breast cancer cells, differential effects were documented on the expression levels of the tumor biologically relevant integrin αvß3 which colocalized with KAI1-WT but not with KAI1-SP. Cellular motility was assessed by video image processing, including motion detection and vector analysis for the quantification and visualization of cell motion parameters. In MDA-MB-231 cells, KAI1-SP provoked a quicker wound gap closure and higher closure rates than KAI1-WT, also reflected by different velocities and average motion amplitudes of singular cells. KAI1-SP induced highest cell motion adjacent to the wound gap borders, whereas in MDA-MB-435 cells a comparable induction of both KAI1 variants was noticed. Moreover, while KAI1-WT reduced cell growth, KAI1-SP signif...Continue Reading

References

Dec 15, 1992·European Journal of Biochemistry·M GurrathR Timpl
Jun 7, 1996·Biochimica Et Biophysica Acta·M E HemlerF Berditchevski
Jun 9, 1998·Japanese Journal of Cancer Research : Gann·A TakaokaK Imai
Jul 31, 1999·The Journal of Cell Biology·F Berditchevski, E Odintsova
May 12, 2000·Nature Cell Biology·D J SiegD D Schlaepfer
Sep 14, 2000·Current Biology : CB·E OdintsovaF Berditchevski
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·T ShinoharaS Sone
Nov 7, 2001·The American Journal of Pathology·F S LiuC H Lu
Jan 5, 2002·The Journal of Cell Biology·M E Hemler
Feb 11, 2003·Trends in Biochemical Sciences·Christopher S StippMartin E Hemler
May 14, 2003·Clinical & Experimental Metastasis·Brunhilde Felding-Habermann
Oct 25, 2003·Journal of Cell Science·Elena OdintsovaFedor Berditchevski
May 26, 2004·Biochemical Society Transactions·S CabodiP Defilippi
Jul 20, 2004·World Journal of Gastroenterology : WJG·De-Hua WuYan-Qing Ding
Nov 8, 2005·Trends in Molecular Medicine·Hélène Tonoli, J Carl Barrett
Feb 21, 2006·Biochemical and Biophysical Research Communications·Bo Keun JeeYoung Lim
Aug 22, 2006·Current Opinion in Cell Biology·Satyajit K Mitra, David D Schlaepfer
Dec 22, 2006·Traffic·Fedor Berditchevski, Elena Odintsova
Feb 21, 2008·World Journal of Gastroenterology : WJG·Jian-Hua XuMin-Pei Liu
Jun 26, 2008·The International Journal of Biochemistry & Cell Biology·Daniela LössnerUte Reuning
Dec 17, 2008·Nature Reviews. Cancer·Margot Zöller
Jan 27, 2009·Experimental and Molecular Pathology·Alaa AfifyLaura Nguyen
Feb 21, 2009·The Biochemical Journal·Charles H Streuli, Nasreen Akhtar
Feb 10, 2010·International Journal of Cancer. Journal International Du Cancer·Amanda Capes-DavisR Ian Freshney
Feb 11, 2010·Journal of Cell Science·Lydia DanglotThierry Galli
Aug 20, 2011·Tissue Engineering. Part C, Methods·Tomohiro HayakawaTeruo Okano
Jul 31, 2013·The Journal of Biological Chemistry·Elena OdintsovaFedor Berditchevski

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Citations

Oct 3, 2019·Scientific Reports·Nikolas HochheimerThorsten Lang
Jun 25, 2020·Cancer Metastasis Reviews·Belinda J Petri, Carolyn M Klinge
Jun 15, 2021·Saudi Journal of Biological Sciences·Khulood M Al-KhaterSuriya Rehman

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