Differential tyrosine phosphorylation of N-methyl-D-aspartate receptor subunits.

The Journal of Biological Chemistry
L F Lau, R L Huganir

Abstract

Protein-tyrosine phosphorylation has recently been suggested to play an important role in synaptic transmission at the neuromuscular junction. The role of tyrosine phosphorylation in the modulation of synaptic function in the central nervous system, however, is not clear. In this study, immunocytochemical staining with an anti-phosphotyrosine antibody demonstrates that there are high levels of phosphotyrosine, which co-localizes with glutamate receptors at excitatory synapses on cultured hippocampal neurons. In addition, the tyrosine phosphorylation of various subtypes of glutamate receptors were examined using subunit-specific antibodies. Glutamate receptors are the major excitatory neurotransmitter receptors in the central nervous system and are classified into three major classes: alpha-amino-3-hydroxy-5-methyl-4-isoxazole proprionate, kainate, and N-methyl-D-aspartate (NMDA) receptors, based on their electrophysiological and pharmacological properties. NMDA receptors play a central role in synaptic plasticity, synaptogenesis, and excitotoxicity and are thought to be heteromeric complexes of the two types of subunits: NR1 and NR2(A-D) subunits. Immunoaffinity chromatography of detergent extracts of rat synaptic plasma membra...Continue Reading

References

Aug 1, 1992·Journal of Neuroscience Research·S H Hanissian, N Sahyoun
Jan 1, 1991·Advances in Experimental Medicine and Biology·R L Huganir
Mar 29, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B G Wallace
Jun 1, 1991·Current Opinion in Neurobiology·K R WagnerR L Huganir
Nov 7, 1991·Nature·K MoriyoshiS Nakanishi
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P F ManessK H Pfenninger
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Sep 11, 1987·Cell·T Hunter
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D T PangP Greengard
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L HuganirP Greengard
Aug 1, 1993·Trends in Pharmacological Sciences·P H Seeburg
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·I S MoonM B Kennedy
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M CraigG Banker
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Jan 7, 1993·Nature·T V Bliss, G L Collingridge

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Citations

Sep 30, 1999·Journal of Neurobiology·I B Black
Dec 4, 2003·Neurobiology of Aging·Lijun LiLynn A Raymond
Oct 17, 1998·Biochemical Pharmacology·M W Salter
Jul 16, 2002·Brain Research. Molecular Brain Research·Anne Marie GuerguerianLee J Martin
Oct 31, 1998·Brain Research. Molecular Brain Research·J E Kraus, J O McNamara
May 13, 1999·Brain Research. Molecular Brain Research·H S Kalluri, M K Ticku
Nov 19, 1997·Neurochemistry International·J W Gurd
Aug 28, 1999·Progress in Neurobiology·T Yamakura, K Shimoji
Aug 1, 1996·Current Opinion in Cell Biology·M D EhlersR L Huganir
Apr 25, 2000·Brain Research. Brain Research Protocols·H HiraiK Yamada
Aug 14, 2009·Neuron Glia Biology·Justin T Rogers, Edwin J Weeber
Mar 23, 2004·Nature Reviews. Neuroscience·Michael W Salter, Lorraine V Kalia
Jul 11, 2013·Nature Reviews. Neuroscience·Anthony J Koleske
Sep 28, 1998·The European Journal of Neuroscience·B Lancaster, M V Rogers
Feb 13, 1999·The European Journal of Neuroscience·Y F LuH Matsui

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