Differential up-regulation of H-2D versus H-2K class I major histocompatibility expression by interferon-gamma: evidence against a trans-acting allele-specific factor

Journal of Interferon Research
R F RichW R Green

Abstract

Previous investigation described a unique differential phenotype for the murine T-cell tumor AKR SL3 with regard to augmentation of class I major histocompatibility complex antigen expression by interferon-gamma (IFN-gamma). Dk expression was increased by IFN-gamma as expected, but Kk expression remained at constitutive levels, despite treatment with a range of doses and times of exposure to IFN-gamma. Analysis of somatic cell hybrids obtained by fusion of AKR SL3 with an H-2Kb and Db IFN-gamma augmentable partner tumor argued against the involvement of locus-specific, trans-acting factors as the basis for the nonaugmentable nature of the Kk gene in AKR SL3. Here, we provide evidence against the remaining possibility of an allele-specific, negative-acting factor in AKR SL3. Hybrids were constructed between drug-marked sublines of AKR SL3 and the R1.G1 T-cell tumor which carries IFN-gamma augmentable Kk and Dk genes. The uniform ability of IFN-gamma to cause substantial increases in the expression of Kk in hybrid populations and a large number of hybrid clones from three separate fusions indicated that a trans-acting, negative factor was not present in AKR SL3. Rather, these data coupled with Northern analysis were consistent wi...Continue Reading

References

Jul 1, 1986·European Journal of Immunology·A Schäfer, W Schmidt
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B Keeney, T H Hansen
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B KorberI Stroynowski
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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