Differential uptake and oxidative stress response in zebrafish fed a single dose of the principal copper and zinc enriched sub-cellular fractions of Gammarus pulex

Aquatic Toxicology
Farhan R KhanChrister Hogstrand

Abstract

The sub-cellular compartmentalisation of trace metals and its effect on trophic transfer and toxicity in the aquatic food chain has been a subject of growing interest. In the present study, the crustacean Gammarus pulex was exposed to either 11 microg Cu l(-1), added solely as the enriched stable isotope 65Cu, or 660 microg Zn l(-1), radiolabeled with 2MBq (65)Zn, for 16 days. Post-exposure the heat stable cytosol containing metallothionein-like proteins (MTLP) and a combined granular and exoskeletal (MRG+exo) fractions were isolated by differential centrifugation, incorporated into gelatin and fed to zebrafish as a single meal. Assimilation efficiency (AE) and intestinal lipid peroxidation, as malondialdehyde (MDA) were measured. There was a significant difference (p<0.05) between the retention of the MTLP-Zn (39.0+/-6.4%) and MRG+exo-Zn (17.2+/-3.7%) and of this zinc retained by the zebrafish a significantly greater proportion of the MTLP-Zn feed had been transported away from the site of uptake. For 65Cu, although the results pointed towards greater bioavailability of the MTLP fraction compared to MRG+exo during the slow elimination phase (24-72 h) these results were not significant (p=0.155). Neither zinc feed provoked a li...Continue Reading

References

May 9, 1998·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·S SarkarD Bhatnagar
Mar 30, 2001·The Journal of Biological Chemistry·K E van HoldeH Decker
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·L A Gaither, D J Eide
Feb 9, 2002·Clinical Science·Linda J HarveySusan J Fairweather-Tait
Nov 29, 2002·The Journal of Experimental Biology·Nicolas R BuryChris N Glover
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology
Oct 19, 2004·Environmental Science & Technology·Martina G VijverWillie J G M Peijnenburg
Oct 28, 2004·Environmental Science & Technology·Marie-Noële CroteauCary B Lopez
Jun 1, 2005·Toxicology and Applied Pharmacology·Mi Ja ChungChrister Hogstrand
Oct 5, 2006·Experimental Biology and Medicine·Mi Ja ChungSung-Joon Lee
Mar 27, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A GeffardO Geffard
Jul 28, 2009·ACS Nano·Pedro J J AlvarezVicki Stone

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Citations

Feb 11, 2012·The Journal of Experimental Biology·Farhan R KhanChrister Hogstrand

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