Differential uptake of chloroquine by human keratinocytes and melanocytes in culture.

Archives of Dermatological Research
G Sjölin-ForsbergO Rollman

Abstract

The antimalarial drug chloroquine has a high affinity for melanin and accumulates in melanin-rich compartments such as those of the eye. Chloroquine is also deposited in cutaneous tissue, but whether the drug distribution is restricted to melanin-producing cells of the skin is not known. In the present study, the uptake of chloroquine by normal human epidermal keratinocytes was compared with that by melanocytes. Selectively cultivated cells were incubated at drug concentrations ranging between 0 and 10000 ng/ml for periods of up to 48 h. Chloroquine was quantified in cells and medium using high performance liquid chromatography and fluorometric detection. In both types of cells there was a rapid uptake of chloroquine within the first 2 h, followed by a slower uptake for 2-6 h until a steady-state condition was reached. Dose dependency was linear, with no sign of saturation, and approximately ten times higher drug concentrations were attained in melanocytes as compared with keratinocytes. No formation of desethylchloroquine, the major systemic metabolite, was detected in either cell type. The observed affinity of chloroquine for normal epidermal melanocytes in vitro suggests that the density and melanogenic activity of skin pigm...Continue Reading

References

Apr 1, 1979·Biochemical Pharmacology·B Larsson, H Tjälve
Aug 1, 1978·Seminars in Arthritis and Rheumatism·E L Dubois
Jul 1, 1977·The British Journal of Dermatology·G Swanbeck, G Wennersten
Jan 1, 1972·Acta Pharmacologica Et Toxicologica·N G Lindquist, S Ullberg
Oct 1, 1973·The Journal of Investigative Dermatology·P L ScholnickH S Marver
Apr 1, 1968·American Journal of Ophthalmology·T LawwillL Altstatt
Dec 21, 1966·Biochimica Et Biophysica Acta·R L O'BrienF E Hahn
Jul 18, 1983·The American Journal of Medicine·A H Mackenzie
Sep 1, 1983·The Journal of Investigative Dermatology·I Rosdahl, H Rorsman
Aug 1, 1993·The Biochemical Journal·O RollmanW J Cunliffe
Jun 1, 1958·The Journal of Investigative Dermatology·B SHAFFERE J LEVY

❮ Previous
Next ❯

Citations

Dec 29, 2010·Indian Journal of Pharmaceutical Sciences·S E AghahowaI M Arhewoh
Jul 29, 2006·The Journal of Pharmacy and Pharmacology·Tracy GarnierSimon L Croft
Jul 4, 2006·The Lancet Oncology·Ashish RustogiRakesh Jalali
Sep 21, 2016·Journal of Labelled Compounds & Radiopharmaceuticals·Molahlehi S SonopoJan R Zeevaart

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.