PMID: 9654333Jul 8, 1998Paper

Differential uptake of dextran beads by astrocytes, macrophages and oligodendrocytes in mixed glial-cell cultures from brains of neonatal rats

Neuroscience Letters
F A Tansey, W Cammer

Abstract

The present study addresses a controversy over the abilities of astrocytes to perform phagocytosis. Primary glial-cell cultures were prepared from the brains of neonatal rats and were incubated with fluorescently-labeled dextran beads (molecular weights approximately 10 and approximately 40 kDa). Astrocytes and oligodendrocytes were double-labeled by immunofluorescence staining of cell-specific markers, and microglia by lectin histochemistry. Cells were permitted to take up beads for 1 h, fixed, and incubated with primary antibodies, followed by fluorescent secondary antibodies or fluorescently-labeled lectin. Macrophages and astrocytes internalized beads of both sizes. In astrocyte processes the beads appeared to line up along glial filaments. The results, which provide direct evidence for uptake of beads by astrocytes in vitro and against equally rapid, if any, uptake by oligodendrocytes, bear upon issues of acid/base balance and glial cell development and are relevant to neuropathological observations in human disease.

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C Chen, M Chesler
Jan 1, 1992·Brain Research. Brain Research Reviews·W E Thomas
Jan 1, 1992·Journal of Neuropathology and Experimental Neurology·N R Ghatak
Sep 1, 1990·The Journal of Cell Biology·R ArmstrongM Dubois-Dalcq
Jan 1, 1990·Progress in Neurobiology·M Chesler
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J Streit
Jan 1, 1990·Acta Neuropathologica·S K Ludwin
Nov 1, 1989·The Journal of Cell Biology·C GodfraindM Dubois-Dalcq
Jan 1, 1984·Journal of Neurochemistry·F C Chiu, J E Goldman
Oct 1, 1993·Neuropathology and Applied Neurobiology·D TroostJ M de Jong
Mar 1, 1993·Journal of Neuropathology and Experimental Neurology·E WuC S Raine

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Citations

Mar 24, 2012·BMC Neuroscience·Josephine PinkernelleGerburg Keilhoff
Aug 27, 2009·PLoS Biology·Won-Suk Chung, Ben A Barres
Dec 24, 2013·Genes & Development·Ozge E Tasdemir-Yilmaz, Marc R Freeman
Feb 22, 2003·The Journal of Comparative Neurology·Ishar DalmauBernardo Castellano
Oct 6, 2015·Fluids and Barriers of the CNS·Beatrice BedussiErik N T P Bakker
Nov 1, 2011·Current Opinion in Neurobiology·Won-Suk Chung, Ben A Barres
Aug 3, 2007·Journal of Biomedical Materials Research. Part a·Roche C de Guzman, Pamela J VandeVord

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