Differential urinary proteins to diagnose coronary heart disease based on iTRAQ quantitative proteomics

Analytical and Bioanalytical Chemistry
Haidan SunYong Zeng

Abstract

Coronary artery disease (CAD) is a manifestation of systemic atherosclerotic disease. It is assessed by intervention or traditional scoring risk factors. Diagnosis is limited by inaccurate and invasive methods. Developing noninvasive methods to screen for the risk of CAD is a major challenge. We aimed to identify urinary proteins associated with CAD. We utilized iTRAQ labeling followed by 2D LC-MS/MS to compare the urinary proteome of CAD patients to healthy cohorts. The multiple reaction monitoring (MRM) was used to verify the differential proteins. ROC analysis based on MRM data was used to evaluate the diagnostic application. A total of 876 proteins were quantified, and 100 differential proteins were found. Functional analysis revealed that the differential proteins were mainly associated with Liver X Receptor/Retinoid X Receptor (LXR/RXR) pathway activation, atherosclerosis signaling, production of nitric oxide and reactive oxygen species, and the top upstream regulator of the differential proteins by IPA analysis indicated to the APOE. Nineteen differential proteins were verified by MRM analysis. ROC based on MRM data revealed that the combination of two proteins (APOD and TFF1) could diagnose CAD with 85% sensitivity and ...Continue Reading

References

Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·Y NakashimaR Ross
Sep 18, 1998·Biochimica Et Biophysica Acta·S RibierasM C Rio
Jan 1, 1950·Cancer·W J YOUDEN
Oct 22, 2004·Molecular & Cellular Proteomics : MCP·Estelle M FachGregory J Opiteck
Nov 26, 2005·The Journal of Clinical Investigation·Somchai ChutipongtanateVisith Thongboonkerd
Dec 13, 2005·Molecular & Cellular Proteomics : MCP·Wei SunYouhe Gao
Jun 9, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·H J SungJ Ko
Oct 24, 2007·Molecular & Cellular Proteomics : MCP·Lukas U ZimmerliAnna F Dominiczak
Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·S Kjellev
Mar 24, 2009·Annual Review of Immunology·Elena Galkina, Klaus Ley
Mar 17, 2010·Experimental Hematology·Cristina BanfiElena Tremoli
Jan 21, 2011·Molecular & Cellular Proteomics : MCP·Fernando de la CuestaFernando Vivanco
Mar 19, 2011·BMC Bioinformatics·Xavier RobinMarkus Müller
Apr 20, 2011·Journal of Proteome Research·Arivusudar MarimuthuAkhilesh Pandey
Apr 28, 2011·Proteomics. Clinical Applications·Christian DellesAnna F Dominiczak
May 17, 2011·Proteome Science·Magdalena LuczakMarek Figlerowicz
May 20, 2011·Nature·Peter LibbyGöran K Hansson
Sep 23, 2011·Molecular and Cellular Biochemistry·Aruna PoduriMadhu Khullar
Nov 9, 2011·Nature Medicine·Christian Weber, Heidi Noels
Oct 16, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Shu Q LiuYu H Wu
Jan 25, 2013·Molecular BioSystems·F RaimondoM Pitto
Feb 23, 2013·Journal of Proteomics·Fernando de la CuestaMaria G Barderas
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kosuke TsukamotoMonty Krieger
Nov 14, 2013·Advanced Biomedical Research·Rajani Kanth VangalaHebbagodi Sridhara
Apr 4, 2014·World Journal of Gastroenterology : WJG·Luise AamannHenning Grønbæk
Mar 20, 2015·Proteomics. Clinical Applications·Catriona E BrownChristian Delles
Apr 19, 2015·Journal of Translational Medicine·Yanbao YuRembert Pieper
Dec 29, 2015·Frontiers in Bioscience (Landmark Edition)·Matthew AminUtpal Sen
Jan 12, 2016·Genomics, Proteomics & Bioinformatics·Manxia An, Youhe Gao
Mar 18, 2016·International Journal of Oncology·Holger HusiJames A Ross
Aug 9, 2016·Cardiovascular Diagnosis and Therapy·Satoshi HondaSatoshi Yasuda

❮ Previous
Next ❯

Citations

Jul 7, 2020·International Journal of Molecular Medicine·Yaqing ZhouEnzhi Jia
May 1, 2021·International Journal of Molecular Sciences·Frederik DesmaraisCatherine Mounier
Jul 3, 2021·Molecular & Cellular Proteomics : MCP·Xiaoyan LiuWei Sun
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.