Jun 30, 2006

Differential usage of non-homologous end-joining and homologous recombination in double strand break repair

DNA Repair
E SonodaShunichi Takeda

Abstract

Repair of DNA double strand breaks (DSBs) plays a critical role in the maintenance of the genome. DSB arise frequently as a consequence of replication fork stalling and also due to the attack of exogenous agents. Repair of broken DNA is essential for survival. Two major pathways, homologous recombination (HR) and non-homologous end-joining (NHEJ) have evolved to deal with these lesions, and are conserved from yeast to vertebrates. Despite the conservation of these pathways, their relative contribution to DSB repair varies greatly between these two species. HR plays a dominant role in any DSB repair in yeast, whereas NHEJ significantly contributes to DSB repair in vertebrates. This active NHEJ requires a regulatory mechanism to choose HR or NHEJ in vertebrate cells. In this review, we illustrate how HR and NHEJ are differentially regulated depending on the phase of cell cycle and on the nature of the DSB.

  • References53
  • Citations229

References

  • References53
  • Citations229

Citations

Mentioned in this Paper

Vertebrates
Saccharomyces cerevisiae Proteins
Embryo
PRKDC gene
Non-Homologous DNA End-Joining
Biochemical Pathway
RAD52 gene
DNA Repair
Plain X-ray
High affinity DNA-binding factor, S cerevisiae

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