Differential use of signal transduction pathways in the gonadotropin-releasing hormone-mediated regulation of gonadotropin subunit gene expression

Endocrinology
B D SaundersU B Kaiser

Abstract

The regulation of LH and FSH subunit gene expression is under the control of GnRH. Physiological changes in the frequency of pulsatile GnRH release from the hypothalamus result in differential stimulation of alpha-, LHbeta-, and FSHbeta-gene expression. Previous studies indicate that the GnRH receptor couples to G proteins of the G(q/11) family, with phosphoinositide turnover and its resultant increase in intracellular calcium concentration and protein kinase C (PKC) activation, to stimulate secretion of LH and FSH. However, the molecular mechanisms by which GnRH mediates its transcriptional effects remain largely unknown. We used GH3 cells, constitutively expressing the rat GnRH receptor (GGH(3)-1' cells) and transiently transfected with a luciferase reporter gene controlled by either the alpha, LHbeta, or FSHbeta gene regulatory region (alphaLUC, LHbetaLUC, and FSHbetaLUC, respectively), to examine the roles of several signal transduction pathways in the GnRH-mediated stimulation of gonadotropin subunit gene expression. Activation of PKC by phorbol, 12-myristate, 13-acetate resulted in an increase in the luciferase activity of all three gonadotropin subunit gene reporter constructs. Phorbol, 12-myristate, 13-acetate had a gre...Continue Reading

Citations

Jul 29, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Johannes D Veldhuis, Cyril Y Bowers
Feb 13, 2003·Molecular and Cellular Endocrinology·Seok Bean SongKyoon Eon Kim
Mar 21, 2001·Bipolar Disorders·P J Goodnick
Dec 24, 2015·Endocrinology·Brian S EdwardsAmy M Navratil
Jul 9, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christian KlausenHamid R Habibi
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H AndoA Urano

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