Differential use of the regulatory elements of the alpha B-crystallin enhancer in cultured murine lung (MLg), lens (alpha TN4-1) and muscle (C2C12) cells

Gene
J I HaynesJ Piatigorsky

Abstract

The mouse alpha B-crystallin-encoding gene (alpha B-cry) is highly expressed in the lens and expressed to lesser extents in other tissues. Here, we investigated alpha B-cry expression in mouse-lung-derived MLg cells. Two sizes of MLg alpha B-cry transcripts comigrated with alpha B-cry transcripts contained in total and poly(A)+RNA from mouse lung, with preference for the larger species in the MLg cells. Expression of both alpha B-cry promoter/cat reporter gene constructs and alpha B-cry enhancer (nt -427/-259)/herpes simplex virus (HSV) thymidine kinase promoter (ptk)/human growth hormone reporter gene (hGH) constructs was studied in transfected MLg cells and the results compared with those obtained from alpha TN4-1 lens and C2C12 muscle cells. The alpha B-cry enhancer increased activity of the endogenous and tk promoters approx. 2-fold in the MLg cells, in contrast to its 3-7-fold effect in alpha TN4-1 cells and 17-20-fold effect in C2C12 myotubes. Site-specific mutagenesis of the previously identified enhancer control elements, alpha B-E-1 (nt -407 to -397), alpha BE-2 (-360 to -327) and MRF (-300 to -288), decreased enhancer strength in transfected MLg cells. DNase I footprinting showed that MLg nuclear proteins occupy only ...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R KlemenzA Aoyama
Mar 1, 1989·Molecular and Cellular Biology·R A DubinJ Piatigorsky
Jan 16, 1989·Biochemical and Biophysical Research Communications·S P Bhat, C N Nagineni
Jan 1, 1988·Annual Review of Biochemistry·G J Wistow, J Piatigorsky
Jun 1, 1974·Journal of Virology·H Yoshikura, Y Hirokawa
Jun 1, 1994·DNA and Cell Biology·A N Srinivasan, S P Bhat
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Kantorow, J Piatigorsky
Apr 11, 1994·Nucleic Acids Research·R Gopal-Srivastava, J Piatigorsky

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Citations

Jul 4, 2012·The International Journal of Biochemistry & Cell Biology·Aurélie de ThonelValérie Mezger
Sep 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J I HaynesJ Piatigorsky
Apr 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J G GeislerM L Mucenski
Dec 1, 1995·Molecular and Cellular Biology·R Gopal-SrivastavaJ Piatigorsky

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