Differential versican isoforms and aggrecan expression in the chicken embryo aorta

The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology
Enrique ArciniegasDavid Parada

Abstract

Members of the family of large chondroitin sulfate proteoglycans (CSPGs), such as versican and aggrecan, are involved in early heart development, and in the development and progression of atherosclerosis and restenosis. Given the important roles played by versican and aggrecan in such processes, we sought to determine whether these molecules are present in the aortic wall during the advanced stages of chicken embryo development and the endothelial-mesenchymal transformation (EMT). Immunolabeling of serial cryosections revealed versican immunoreactivity around the cells within the intimal thickening, and the cells organized in lamellar and interlamellar cell layers. In contrast, a weak aggrecan immunoreactivity was limited to the cells arranged into lamellar and interlamellar cell layers. Immunolabeling also demonstrated that V2 is the main versican isoform present at the intimal thickening. According to immunoblotting analysis, the aggrecan content was very low in all stages examined, and two versican isoforms (V0 and V2) were present at day 14 of development. We also investigated whether versican isoforms were present during EMT in vitro. Versican immunoreactivity was detected in patches of endothelial cells; in the detaching ...Continue Reading

References

Dec 15, 1992·The Biochemical Journal·L Chandrasekaran, M L Tanzer
Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
Jan 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J K ChenW L McKeehan
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J K ChenW L McKeehan
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·L Y YaoL J Sandell
Jun 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J M SorreliA I Caplan
Jul 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y NakajimaR R Markwald
Jun 23, 1998·The Journal of Biological Chemistry·M SchmalfeldtD R Zimmermann
Jan 30, 1999·Anatomy and Embryology·J M SorrellA I Caplan
Jul 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·J M LemireT N Wight
Aug 27, 1999·Anatomy and Embryology·A KohlerA Draeger
Dec 22, 1999·The Anatomical Record·E ArciniegasR G Carlini
Nov 22, 2001·The Journal of Biological Chemistry·Bryan P TooleMarkku I Tammi
Sep 17, 2002·Current Opinion in Cell Biology·Thomas N Wight
Oct 12, 2002·Developmental Biology·A GatoC Martínez-Alvarez
Dec 21, 2002·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Enrique ArciniegasArmando Graterol

❮ Previous
Next ❯

Citations

Feb 3, 2006·Molecular Biology of the Cell·Wang ShengBurton B Yang
Jul 9, 2009·Physiological Reviews·Jessica E Wagenseil, Robert P Mecham
Dec 29, 2011·European Journal of Histochemistry : EJH·L M CarrilloR Ramírez
Jul 13, 2006·Differentiation; Research in Biological Diversity·Enrique ArciniegasLuz M Carrillo
Oct 27, 2006·Developmental Biology·Elaine L Shelton, Katherine E Yutzey
Sep 2, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Christopher D KochSuneel S Apte
Jun 25, 2020·American Journal of Physiology. Cell Physiology·Zihan MaWei Kong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.