Differentially expressed genes in the prostate cancer cell line LNCaP after exposure to androgen and anti-androgen

Cancer Genetics and Cytogenetics
Cláudia M Coutinho-CamilloMaria A Nagai

Abstract

Androgens play an important role in growth and maintenance of prostate cells. The actions of androgens are mediated by the androgen receptor (AR), a transcription factor member of the super-family of nuclear hormone receptors. Androgen regulated genes (ARGs) are potential markers for early diagnosis and treatment of prostate cancer patients. In the present study, we used DDRT-PCR (differential display reverse transcriptase polymerase chain reaction) technique in order to investigate differentially expressed genes in the prostate cancer cell line LNCaP after treatment with dihydrotestosterone and bicalutamide for 6, 24, and 48 hours. Fifty-five differentially expressed fragments were isolated, cloned, and sequenced. Sequencing analysis of these fragments revealed 56 different transcripts that showed homology to transcription factors, cell cycle regulators, metabolic enzymes, and hypothetical proteins. Among the differentially expressed genes, SPA17 and DDEF2 were further validated using quantitative real time RT-PCR (qPCR) in a series of 25 prostate tumor samples. The DDEF2 gene is involved in adhesion and cell migration of monocytes, and the SPA17 gene might be involved in cellular signal transduction. The transcripts of both, ...Continue Reading

References

Jul 17, 1997·International Journal of Cancer. Journal International Du Cancer·R DahiyaG Deng
Feb 10, 1998·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·K IshikawaO Ohara
Jun 30, 1998·Virchows Archiv : an International Journal of Pathology·A Latil, R Lidereau
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SrikantanS Srivastava
Feb 15, 2001·Cancer Genetics and Cytogenetics·J J JordanJ V Tricoli
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·L L XuS Srivastava
Jun 21, 2001·The Biochemical Journal·Y WenM G O'Rand
May 15, 2002·Genes, Chromosomes & Cancer·Eija-Riitta HyytinenJin-Tang Dong
May 17, 2002·The Journal of Biological Chemistry·David MasielloSteven P Balk
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peter S NelsonBiaoyang Lin
Sep 6, 2002·Cancer Letters·Ann De JongDick Robbins
Oct 11, 2002·Nature·Sooryanarayana VaramballyArul M Chinnaiyan
Dec 24, 2002·The Prostate·Hideyasu MatsuyamaPeter Ekman
Jan 29, 2003·Cancer Genetics and Cytogenetics·Ratna ChakrabartiMegan Muroski
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Hitoshi IshiguroYoshinobu Kubota
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eric S MartinCarlo M Croce
Jan 9, 2004·International Journal of Cancer. Journal International Du Cancer·J Michael StraughnTheresa V Strong
Feb 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rolf von KnoblochRainer Hofmann
May 8, 2004·Endocrinology·Anne Marie VelascoYixian Zhang
Oct 12, 2004·Cancer Genetics and Cytogenetics·Bernd WullichVolker Jung
Dec 16, 2004·Molecular Carcinogenesis·Shilpi AroraRanju Ralhan
Feb 18, 2005·The Prostate·Takeshi UedaTomohiko Ichikawa

❮ Previous
Next ❯

Citations

Oct 18, 2006·The Prostate·Maria ViasIan G Mills
May 1, 2007·Cellular & Molecular Biology Letters·Wenwen LiuAnli Jiang
Jun 15, 2016·Journal of Dermatological Science·Michael SandSchapoor Hessam
Feb 20, 2015·Molecular & Cellular Proteomics : MCP·Greg N BrookeCharlotte L Bevan
Dec 18, 2007·Molecular Biology Reports·Weiwen ChenJianye Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.