Differentially Expressed Intrahepatic Genes Contribute to Control of Hepatitis B Virus Replication in the Inactive Carrier Phase

The Journal of Infectious Diseases
Hongyan LiuJiming Zhang

Abstract

The natural history of chronic hepatitis B virus (HBV) infection was divided into 4 phases. Patients in the inactive carrier (IC) status and immune tolerant (IT) phase had normal alanine aminotransferase levels but huge different viral loads. The mechanism underlying low viral replication status in IC phase is unknown. We determined the intrahepatic transcriptomes of 83 chronic hepatitis B patients by microarray analysis of liver biopsies, and screened the effect of differentially regulated genes on HBV replication using specific small interfering RNAs in vitro. The gene profile distinguishing active chronic hepatitis from IT and IC was predominantly composed of immune-related genes. The liver transcriptomes between the IT and IC phase were largely similar, and 109 expressed genes were significantly different. By performing systematic screening, 5 candidate genes including EVA1A, which were expressed at a relative higher level in IC phase than IT, were identified to regulate HBV replication and gene expression in cellular models. The immune-related pathways were up-regulated in the active chronic hepatitis phase but not in the IT and IC phase. A number of intrahepatic genes highly expressed in the IC phase may participate in th...Continue Reading

References

Nov 26, 2003·Antiviral Research·A BertolettiRoger Williams
Apr 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Stefan WielandFrancis V Chisari
May 14, 2005·Journal of Virology·Masanori IsogawaFrancis V Chisari
Jan 21, 2006·Nature Immunology·Taro Kawai, Shizuo Akira
May 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Lan WangYingyu Chen
Oct 12, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun WuJoerg F Schlaak
Nov 28, 2007·Annual Review of Pathology·Luca G Guidotti, Francis V Chisari
Aug 13, 2008·The Journal of Experimental Medicine·Abhishek DasMala K Maini
Feb 12, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Yun-Fan Liaw
Apr 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brian J McMahon
Apr 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chien-Jen ChenUNKNOWN REVEAL-HBV Study Group
Mar 20, 2010·Digestive Diseases and Sciences·Yun-Fan LiawEdward Gane
Mar 23, 2010·Autophagy·Donna SirJing-Hsiung James Ou
Oct 1, 2011·Journal of Virology·Yongjun TianJing-Hsiung James Ou
Jul 1, 2010·Viruses·Malika Ait-GoughoulteDavid Durantel
Nov 15, 2011·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Jan 25, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ning LiWei Liu
Mar 23, 2012·Journal of Hepatology·UNKNOWN European Association For The Study Of The Liver
Apr 13, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Dong XuYing Yu Chen
May 24, 2012·Journal of Hepatology·Xiaoyong ZhangMengji Lu
Jun 20, 2012·Gastroenterology·Patrick T F KennedyAntonio Bertoletti
Jul 9, 2013·Nature Immunology·Jianhua LiZhenghong Yuan
Jul 23, 2013·Virology Journal·Wolfram H Gerlich
Mar 27, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Thomas VanwolleghemAndre Boonstra
Dec 20, 2015·Gastroenterology·Jang-June ParkUNKNOWN HBRN

❮ Previous
Next ❯

Citations

Oct 23, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ejuan ZhangMengji Lu
Apr 26, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jieliang ChenZhenghong Yuan
Jun 12, 2021·Frontiers in Immunology·Xufeng LuoRuth Broering
Jul 20, 2021·The Journal of Infectious Diseases·Noe Rico MontanariAndre Boonstra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.