Differentially expressed protein profile of human dental pulp cells in the early process of odontoblast-like differentiation in vitro

Journal of Endodontics
Xi WeiYin Xiao

Abstract

Dental pulp cells (DPCs) are capable of differentiating into odontoblasts that secrete reparative dentin after pulp injury. The molecular mechanisms governing reparative dentinogenesis are yet to be fully understood. Here we investigated the differential protein profile of human DPCs undergoing odontogenic induction for 7 days. Using two-dimensional differential gel electrophoresis coupled with matrix-assisted laser adsorption ionization time of flight mass spectrometry, 23 protein spots related to the early odontogenic differentiation were identified. These proteins included cytoskeleton proteins, nuclear proteins, cell membrane-bound molecules, proteins involved in matrix synthesis, and metabolic enzymes. The expression of four identified proteins, which were heteronuclear ribonuclear proteins C, annexin VI, collagen type VI, and matrilin-2, was confirmed by Western blot and real-time real-time polymerase chain reaction analyses. This study generated a proteome reference map during odontoblast-like differentiation of human DPCs, which will be valuable to better understand the underlying molecular mechanisms in odontoblast-like differentiation.

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Citations

Sep 17, 2013·Journal of Cellular Physiology·Taia M B RezendeOctávio L Franco
May 18, 2016·International Journal of Molecular Sciences·Zohaib KhurshidIhtesham Ur Rehman
Aug 16, 2016·Experimental Cell Research·Katsuhiro UzawaHideki Tanzawa
Oct 30, 2016·Journal of Cellular Physiology·Jie LiJinlin Song
Mar 30, 2010·Journal of Dental Research·C Morsczeck, G Schmalz
Aug 4, 2016·Molecular Medicine Reports·Chanchan ChenHaijing Gu
Nov 13, 2020·World Journal of Stem Cells·Jagadish HosmaniHarish C Chandramoorthy

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