Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome

Clinical and Experimental Nephrology
C P SureshAshish Datt Upadhyay

Abstract

We performed a discovery phase of urinary proteomic profile in children with idiopathic nephrotic syndrome and validated selected biomarkers. Urinary proteomic profile was performed using isobaric tags for relative and absolute quantitation labeling, coupled with liquid chromatography-matrix assisted laser desorption and ionization analysis. Validation of biomarkers apolipoprotein A1, alpha 2 macroglobulin, orosomucoid 2, retinol binding protein 4 and leucine-rich alpha 2-glycoprotein 1 was done by enzyme-linked immunosorbent assay. Apolipoprotein A1 levels of <0.48 µg/mg of creatinine-differentiated steroid-resistant nephrotic syndrome (SRNS) from first episode nephrotic syndrome, area under curve (AUC) [0.99 (CI 0.9-1.0), 100 % sensitivity and 100 % specificity] and a value of <0.24 µg/mg of creatinine could differentiate SRNS from frequently relapsing nephrotic syndrome/steroid dependent nephrotic syndrome [AUC 0.99 (CI 0.9-1.0), 100 % sensitivity and 100 % specificity]. Alpha 2 macroglobulin could differentiate children with SRNS-focal segmental glomerulosclerosis (FSGS) from SRNS-minimal change disease (MCD) at values >3.3 µg/mg of creatinine [AUC 0.84 (CI 0.62-1.0), 90 % sensitivity and 85 % specificity]. Orosomucoid 2 >1...Continue Reading

References

Aug 1, 1990·Archives of Internal Medicine·S M Grundy, G L Vega
Aug 1, 1989·Journal of the American College of Nutrition·S K Mahajan
Jan 1, 1989·Nephron·N TümerM Ekim
Dec 22, 1983·The New England Journal of Medicine·J M GinsbergS Garella
Jul 1, 1995·The Tohoku Journal of Experimental Medicine·S ItoA Shibata
Jun 1, 1997·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·P A TomlinsonC Chantler
Aug 5, 1998·Kidney International·M G de Sain-van der VeldenG A Kaysen
Nov 12, 1998·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S C DillonV Shah
Nov 4, 2000·Biochimica Et Biophysica Acta·T FournierD Porquet
Mar 13, 2003·Kidney International·Giuseppe D'Amico, Claudio Bazzi
Apr 5, 2003·Kidney International·Nosratola D Vaziri
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Dec 8, 2004·Journal of the American Society of Nephrology : JASN·Edmond O'RiordanMichael S Goligorsky
May 11, 2005·Kidney International·Marion HaubitzHarald Mischak
Jun 18, 2005·American Journal of Nephrology·Mai T NguyenPrasad Devarajan
Oct 21, 2005·Proteomics·Katherine R KozakRobin Farias-Eisner
Jan 18, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Clara HjalmarssonBörje Haraldsson
Feb 16, 2007·Journal of the American Society of Nephrology : JASN·Sanju A VargheseJohn M Arthur
Mar 17, 2007·Hematology·Marco Zaffanello, Massimo Franchini
Aug 30, 2008·Lipids in Health and Disease·Simone K FreyFlorian J Schweigert
Feb 5, 2010·Journal of Biomedicine & Biotechnology·Todd H CorzettBrett A Chromy
Jul 1, 2008·Proteomics. Clinical Applications·Giovanni CandianoGian Marco Ghiggeri
Aug 29, 2012·Proteomics. Clinical Applications·René Frydensbjerg AndersenSøren Rittig
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan
Dec 5, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J Lopez-HellinUNKNOWN GREAT Study Group
May 3, 2014·Advances in Clinical Chemistry·Anthony G W NordenRobert J Unwin
Jul 18, 2014·Nephro-urology Monthly·Shiva KalantariRoman Zubarev

❮ Previous
Next ❯

Citations

Nov 13, 2015·Analytical Chemistry·Janice MayneDaniel Figeys
Jun 3, 2016·Frontiers in Pediatrics·Rebecca HjortenKimberly Jean Reidy
Sep 15, 2019·Journal of Medical Genetics·Ivan D DoykovKevin Mills
Aug 9, 2016·Expert Review of Proteomics·Gavino FaaMassimo Castagnola
Oct 28, 2019·Nature Reviews. Nephrology·Moin A Saleem
Jan 4, 2021·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Gabriel Cara-Fuentes, William E Smoyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.