Differentially Methylated Genes in Saliva are linked to Childhood Stress

Scientific Reports
Ligia A PapaleReid S Alisch

Abstract

Chronic and severe stress exposure in early childhood is associated with the development of psychiatric disorders. Yet, the molecular mechanisms underlying this relationship remain poorly understood. Here, we profile molecular marks (DNA methylation and gene expression) throughout the human genome to determine the associations between childhood stress exposure and gene regulation. To do so, we collected saliva tissue from prepubertal girls (mean age 10.9 ± 1.26 years) who had experienced different levels of childhood adversity, ranging from mild to severe. We found 122 differentially methylated genes (FDR P-value < 0.05) associated with high childhood stress exposures that affect brain development. Of these differentially methylated genes, 12 also differed in gene expression. To further investigate the potential effects of stress exposure on gene regulation, we examined the DNA sequences flanking all the differentially methylated loci. This analysis revealed enrichment of known binding sites for transcription factors, suggesting that DNA methylation may regulate gene expression by mediating transcription factor binding on these genes. Together, these findings indicate a possible neuromolecular mechanism linking children's socia...Continue Reading

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Citations

Feb 20, 2020·International Journal of Environmental Research and Public Health·Hannah Lui Park
Jul 25, 2020·Epigenomics·Bernardo J KrauseJames Hamilton
Dec 18, 2020·Journal of Neurodevelopmental Disorders·Karen E Smith, Seth D Pollak
May 10, 2021·Clinical Epigenetics·Faten Taki, Inmaculada de Melo-Martin
Jun 26, 2021·The European Journal of Neuroscience·Jacqueline S WomersleySîan M J Hemmings

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Datasets Mentioned

BETA
GSE112314

Software Mentioned

DREME suite
SpaMo
Bowtie
R package limma
DREME
R package minfi
R package clusterProfiler
R package NHMMfdr
R package sva
R package preprocessCore

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