Differentially Regulated Transcription Factors and ABC Transporters in a Mitochondrial Dynamics Mutant Can Alter Azole Susceptibility of Aspergillus fumigatus

Frontiers in Microbiology
Laura SturmJohannes Wagener

Abstract

Azole resistance of the fungal pathogen Aspergillus fumigatus is an emerging problem. To identify novel mechanisms that could mediate azole resistance in A. fumigatus, we analyzed the transcriptome of a mitochondrial fission/fusion mutant that exhibits increased azole tolerance. Approximately 12% of the annotated genes are differentially regulated in this strain. This comprises upregulation of Cyp51A, the azole target structure, upregulation of ATP-binding cassette (ABC) superfamily and major facilitator superfamily (MFS) transporters and differential regulation of transcription factors. To study their impact on azole tolerance, conditional mutants were constructed of seven ABC transporters and 17 transcription factors. Under repressed conditions, growth rates and azole susceptibility of the mutants were similar to wild type. Under induced conditions, several transcription factor mutants showed growth phenotypes. In addition, four ABC transporter mutants and seven transcription factor mutants exhibited altered azole susceptibility. However, deletion of individual identified ABC transporters and transcription factors did not affect the increased azole tolerance of the fission/fusion mutant. Our results revealed the ability of mu...Continue Reading

References

Sep 2, 2011·European Respiratory Review : an Official Journal of the European Respiratory Society·M KoushaA O Soubani
Oct 5, 2011·Essays in Biochemistry·Cornelia KleinMartin Valachovic
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
Apr 13, 2013·The Journal of Antimicrobial Chemotherapy·Marcin G FraczekPaul Bowyer
Jan 1, 2014·G3 : Genes - Genomes - Genetics·Stacia R EngelJ Michael Cherry
Jun 7, 2014·Frontiers in Physiology·Catarina CostaMiguel C Teixeira
Oct 31, 2014·Thorax·Chris Kosmidis, David W Denning
Nov 12, 2014·Cold Spring Harbor Perspectives in Medicine·Leah E CowenDavid S Perlin
May 20, 2015·Emerging Infectious Diseases·J W M van der LindenP E Verweij
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
Jan 2, 2016·Advances in Experimental Medicine and Biology·Rajendra PrasadAbdul Haseeb Shah
Aug 3, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas F PattersonJohn E Bennett
Aug 5, 2017·The Lancet Infectious Diseases·David S PerlinAna Alastruey-Izquierdo
May 24, 2018·Nucleic Acids Research·Enis AfganDaniel Blankenberg
Aug 29, 2018·Journal of Fungi·Evelina Y BasenkoChristiane Hertz-Fowler
Oct 12, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pieter P LestradePaul E Verweij

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Methods Mentioned

BETA
electrophoresis
PCR
reverse transcription PCR

Software Mentioned

Knime
Cuffdiff
InView Transcriptome Explorer
GraphPad Prism
Galaxy

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Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.