Differentiated osteoblasts derived decellularized extracellular matrix to promote osteogenic differentiation

Biomaterials Research
Jin JeonHee Seok Yang

Abstract

The extracellular matrix (ECM) can directly or indirectly influence on regulation of cell functions such as cell adhesion, migration, proliferation and differentiation. The cell derived ECM (CD-ECM) is a useful in vitro model for studying the comprehensive functions of CD-ECM because it maintains a native-like structure and composition. In this study, the CD-ECM is obtained and a test is carried out to determine the effectiveness of several combinations of decellularized methods. These methods were used to regulate the optimal ECM compositions to be induced by osteogenic differentiation using primary isolated osteoblasts. We investigated the effect of osteoblasts re-seeded onto normal osteoblast ECM under the growth medium (GM-ECM) and the osteogenic differentiation medium (OD-ECM). The osteoblasts were then cultured statically for 1, 2, and 4 weeks in a growth medium or differentiation medium. Before osteoblast culture, we performed immunostaining with filamentous actin and nuclei, and then performed DNA quantification. After each culture period, the osteogenic differentiation of the osteoblasts re-seeded on the OD-ECMs was enhanced osteogenic differentiation which confirmed by alkaline phosphatase staining and quantification,...Continue Reading

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Citations

Feb 26, 2020·Cells·Agnieszka SmieszekKrzysztof Marycz
Jan 2, 2019·Biomaterials Research·Seong Gyu KwonJae Ho Kim
Oct 17, 2019·Journal of Biomedical Materials Research. Part a·Radoslaw JunkaXiaojun Yu
Oct 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Cininta SavitriKwideok Park
Oct 20, 2020·Biomaterials Science·Yuting FengGuoli Yang
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Marisa AssunçãoAnna Blocki
Apr 3, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·Nadia GaribaldiRoberta Besio
May 8, 2021·Biomaterials Research·Yoon Young GoJae-Jun Song
Jan 19, 2021·ACS Biomaterials Science & Engineering·Weilong XieChangren Zhou

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