Abstract
Monoclonal antibodies (MAbs) against Vibrio vulnificus (isolate I, VVC and isolate II, VVB) were raised using heat-killed and heat-killed plus SDS-mercaptoethanol treated forms of VVC and VVB for immunizing Swiss mice. Twenty three hybridomas producing MAbs against V. vulnificus were selected and divided into five groups according to their specificities to different V. vulnificus isolates and apparent protein antigens which ranged from approximately 3-50 kDa. Four groups were specific to V. vulnificus without cross reactivity to either other Vibrio spp. or other bacterial species. In dot blot based assays, one group of MAbs were specific to VVC, with a sensitivity of approximately 1.6 x 10(7) CFU ml(-1) (approximately 1.6 x 10(4) cells spot(-1)), and bound to proteins of approximately 50 and approximately 39 kDa. Other MAbs, binding to proteins ranging from approximately 3-14 and approximately 40 kDa, detected VVB (but not VVC) with high sensitivity at approximately 1.6 x 10(5) and 4 x 10(6) CFU ml(-1) (approximately 1.6 x 10(2) and 4 x 10(3) cells spot(-1)), respectively. In addition, certain MAbs were able to recognize V. vulnificus in tissues by means of immunohistochemistry. The remaining groups demonstrated cross reactivit...Continue Reading
References
Jul 1, 1979·European Journal of Immunology·T R MosmannA R Williamson
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Mar 1, 1989·Archives of Ophthalmology·M DiGaetanoJ G Straus
Aug 1, 1987·Journal of General Microbiology·K OkadaK Amako
Aug 15, 1970·Nature·U K Laemmli
May 26, 1999·FEMS Microbiology Letters·D A Linkous, J D Oliver
Sep 15, 2000·Diseases of Aquatic Organisms·P SithigorngulP Menasveta
Jul 28, 2001·Journal of Applied Microbiology·M Cerdà-CuéllarA R Blanch
Jun 22, 2002·International Journal of Food Microbiology·John Sumner, Thomas Ross
Jan 9, 2003·Journal of Clinical Microbiology·William B NilssonMark S Strom
Jun 6, 2003·Fish & Shellfish Immunology·M D Esteve-GassentC Amaro
Jan 14, 2004·Microbiological Research·Baskaralingam Vaseeharan, Palaniappan Ramasamy
Jul 28, 2004·Fish & Shellfish Immunology·M Dolores Esteve-Gassent, Carmen Amaro
Oct 19, 2004·Journal of Microbiological Methods·Valerie J HarwoodAnita C Wright
Feb 9, 2005·Diagnostic Microbiology and Infectious Disease·John MidturiRobert Fader
Apr 12, 2005·Diseases of Aquatic Organisms·Wannipa PhianphakPaisarn Sithigorngul
Jun 21, 2005·Epidemiology and Infection·J D Oliver
May 1, 2004·FEMS Microbiology Ecology·Hiroshi Fukushima, Ryotaro Seki