Differentiating between isolates of Vibrio vulnificus with monoclonal antibodies

Journal of Microbiological Methods
Sirirat RengpipatSombat Rukpratanporn

Abstract

Monoclonal antibodies (MAbs) against Vibrio vulnificus (isolate I, VVC and isolate II, VVB) were raised using heat-killed and heat-killed plus SDS-mercaptoethanol treated forms of VVC and VVB for immunizing Swiss mice. Twenty three hybridomas producing MAbs against V. vulnificus were selected and divided into five groups according to their specificities to different V. vulnificus isolates and apparent protein antigens which ranged from approximately 3-50 kDa. Four groups were specific to V. vulnificus without cross reactivity to either other Vibrio spp. or other bacterial species. In dot blot based assays, one group of MAbs were specific to VVC, with a sensitivity of approximately 1.6 x 10(7) CFU ml(-1) (approximately 1.6 x 10(4) cells spot(-1)), and bound to proteins of approximately 50 and approximately 39 kDa. Other MAbs, binding to proteins ranging from approximately 3-14 and approximately 40 kDa, detected VVB (but not VVC) with high sensitivity at approximately 1.6 x 10(5) and 4 x 10(6) CFU ml(-1) (approximately 1.6 x 10(2) and 4 x 10(3) cells spot(-1)), respectively. In addition, certain MAbs were able to recognize V. vulnificus in tissues by means of immunohistochemistry. The remaining groups demonstrated cross reactivit...Continue Reading

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Citations

Feb 1, 2010·Environmental Microbiology Reports·Anita MantiWally Baffone
Mar 17, 2010·Journal of Agricultural and Food Chemistry·Maria-Jose ChapelaJuan M Vieites

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