PMID: 20650109Aug 1, 1995Paper

Differentiating embryonic stem cells as an in vitro model of early erythropoiesis

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M GassmannC Bauer

Abstract

Extended studies on mouse embryos during the earliest stages of development are limited owing to both the large number of preimplantation embryos usually required and the difficulties in manipulating embryos after implantation; an in vitro model for embryogenesis would therefore be useful. Recently, blastocyst-derived mouse embryonic stem (ES) cells have been reported to differentiate in vitro to generate embryoid bodies (EBs) which form endoderm, mesoderm and ectoderm, thereby partially mimicking early stages of murine development. After 7 days of differentiation, EBs consisted of 6-10 x 10(3) cells. Confocal laser scanning microscopy of EBs after 13-14 days of differentiation (diameter 650-800 mum) revealed that they were vital, whereas similar-sized human malignant glioma (U 343 MG) cell spheroids showed cell death in the core region. To evaluate the potential of this system as an in vitro model of embryonic erythropoiesis, the EBs were analysed for the presence of erythropoietin (EPO) mRNA by reverse transcriptase-mediated polymerase chain reaction (RT-PCR) and we adapted a 2,7-diaminofluorene (DAF)-based staining method to detect haemoglobin in situ. DAF-positive cells appeared at day 5-6 of differentiation, whereas EPO mR...Continue Reading

References

Apr 1, 1992·Physiological Reviews·W Jelkmann
Jan 1, 1992·Annual Review of Immunology·B H Koller, O Smithies
Jul 1, 1992·Journal of Cellular Biochemistry·H R SnodgrassE Bruyns
Dec 15, 1990·The Biochemical Journal·A R CrossH Acker
Mar 1, 1986·Molecular and Cellular Biology·C B Shoemaker, L D Mitsock
Jan 1, 1984·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·H Acker
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·P MountfordA Smith
Jan 1, 1993·Molecular and Cellular Biology·G KellerM V Wiles

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Citations

Apr 1, 1996·The European Journal of Neuroscience·H H MartiM Gassmann
Feb 11, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S BichetM Gassmann

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