Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms

Journal of Clinical Microbiology
Makeda SemretMarcel A Behr

Abstract

The Mycobacterium avium species consists of a group of organisms that are genetically related but phenotypically diverse, with certain variants presenting clear differences in terms of their host association and disease manifestations. The ability to distinguish between these subtypes is of relevance for accurate diagnosis and for control programs. Using a comparative genomics approach, we have uncovered large sequence polymorphisms that are, respectively, absent from bird-type M. avium isolates and from cattle types and sheep types of M. avium subsp. paratuberculosis. By evaluating the distribution of these genomic polymorphisms across a panel of strains, we were able to assign unique genomic signatures to these host-associated variants. We propose a simple PCR-based strategy based on these polymorphisms that can rapidly type M. avium isolates into these subgroups.

References

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Aug 6, 2005·Journal of Clinical Microbiology·Makeda SemretMarcel A Behr
Feb 4, 2006·Journal of Clinical Microbiology·Christine Y TurenneMarcel A Behr

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Citations

Mar 1, 2009·Indian Journal of Microbiology·Varalakshmi D VissaPatrick J Brennan
Oct 29, 2011·Journal of Microbiological Methods·Pierre E DouarreJim M O'Mahony
Jul 27, 2010·Epidemiology and Infection·J ÁlvarezL de Juan
Oct 3, 2006·Inflammatory Bowel Diseases·Marcel A Behr, Erwin Schurr
Jan 16, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Rachel M Thomson, Wing-Wai Yew
Oct 20, 2009·Applied and Environmental Microbiology·James CarrollJim O'Mahony
Apr 13, 2007·Clinical Microbiology Reviews·Christine Y TurenneMarcel A Behr
Jan 16, 2009·Journal of Clinical Microbiology·Nick MackenzieJeroen M De Buck
Mar 7, 2006·Journal of Clinical Microbiology·Makeda SemretMarcel A Behr
Jan 17, 2009·BMC Genomics·Chia-wei WuAdel M Talaat
Oct 15, 2009·Future Microbiology·Marcel A Behr, Joseph O Falkinham
Jan 17, 2012·The Veterinary Clinics of North America. Exotic Animal Practice·Mark D Schrenzel
Jun 21, 2011·Research in Veterinary Science·Elena CastellanosAlicia Aranaz
Aug 4, 2009·Veterinary Microbiology·Petra MöbiusHeike Köhler
Dec 19, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laura Rindi, Carlo Garzelli
Feb 20, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E CastellanosJ De Buck
Mar 9, 2012·Veterinary Microbiology·Valerie HughesKaren Stevenson
Oct 8, 2008·Veterinary Microbiology·Jagdeep Singh SohalSwati Subodh
Dec 30, 2015·Journal of Bacteriology·Joyce WangMarcel A Behr
Sep 9, 2006·Clinical and Vaccine Immunology : CVI·Makeda SemretMarcel A Behr
Nov 26, 2008·Journal of Bacteriology·David C AlexanderMarcel A Behr
Feb 15, 2020·Microbiology Resource Announcements·Ralph GoetheBoyke Bunk

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