PMID: 6403505Apr 1, 1983

Differentiation after premature release of intraperiplasmically growing Bdellovibrio bacteriovorous

Journal of Bacteriology
E G Ruby, S C Rittenberg

Abstract

Bdellovibrio bacteriovorous attacks and penetrates other gram-negative bacteria, creating a growth chamber termed a bdelloplast. We have found that exposing the bdelloplasts to EDTA, followed by treatment with a lytic enzyme concentrate derived from bdellovirio cultures, prematurely released the intraperiplasmically growing bdellovibrios at any time during their growth cycle. Upon release, the growth-form bdellovibrios terminated any initiated rounds of DNA synthesis and differentiated into motile attack-form cells. The ability of growth-form cells to synthesize DNA appears to depend upon an initiation signal that is not received until about 60 min after attack. Each subsequent round of DNA synthesis by the growing bdellovibrio filaments seems to require an additional initiation signal that is provided by their intraperiplasmic environment. Differentiation included fragmentation into multiple progeny cells to a degree proportional to the extent of intraperiplasmic growth. This differentiation could be performed totally at the expense of cellular reserves. The significance of these data to an understanding of the regulation of differentiation in bdellovibrios is discussed.

Citations

Jan 1, 1989·Archives of Microbiology·K M Gray, E G Ruby
Oct 19, 2010·Journal of Bacteriology·Alan J Wolfe
Jan 11, 2011·Molecular Oral Microbiology·M Van EsscheW Teughels
Jul 31, 2007·Future Microbiology·Eckhard StrauchMichael Linscheid
Mar 1, 2012·BMB Reports·Mohammed DwidarRobert J Mitchell
Oct 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Or RotemEdouard Jurkevitch
Feb 22, 2017·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Juan A Arias Del AngelMariana Benítez

Related Concepts

Bdellovibrio
DNA, Bacterial
Edetic Acid, Sodium Salt

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