Differentiation and function of osteoclasts cultured on bone and cartilage

Journal of Electron Microscopy
Reina SuzumotoTakahisa Sasaki

Abstract

We examined the differentiation and resorptive function of osteoclasts (OC) cultured on the slices of calcified bone, decalcified bone and hyaline cartilage, and found that OC differentiation depends on the co-cultured substratum, as well as osteoblast-derived factors. Bone marrow-derived macrophages (BMM) were formed from marrow cells of 5 week old ddY mice and cultured for 3 days on freeze-dried slices of calcified bone, decalcified bone or cartilage, all prepared from rabbit costal bone. BMM cultured on calcified bone slices exhibited tartrate-resistant acid phosphatase (TRAP) activity and were structurally characterized by multinucleation and ruffled border development. However, on decalcified bone slices, BMM seldom became multinucleated and exhibited weak TRAP activity. BMM cultured on cartilage slices were mononuclear, devoid of TRAP activity and structurally resembled mononuclear phagocytes. In SEM observations of co-cultured slices, resorption lacunae were formed only on calcified bone slices, and not on slices of decalcified bone and cartilage. Our results, therefore, indicated that BMM could differentiate into functional OC only on calcified bone slices, suggesting a key role of calcified components in the bone matri...Continue Reading

Citations

Aug 4, 2009·Biomaterials·Gemma L JonesSarah H Cartmell
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Christian RichardDavid Goltzman
Sep 23, 2014·The Journal of Steroid Biochemistry and Molecular Biology·D Goltzman
Jul 12, 2012·Virchows Archiv : an International Journal of Pathology·H J KnowlesN A Athanasou

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