Differentiation and identification of Shigella spp. and enteroinvasive Escherichia coli in environmental waters by a molecular method and biochemical test

Water Research
Bing-Mu HsuFeng-Cheng Shih

Abstract

Both Shigella spp. and enteroinvasive Escherichia coli (EIEC) are important human pathogens that are responsible for the majority of cases of endemic bacillary dysentery. However, they are difficult to identify and differentiate by biochemical tests or molecular methods alone. In this study, we developed a procedure to detect Shigella spp. and EIEC from environmental water samples using membrane filtration followed by nutrient broth enrichment, isolation using selective culture plates, and identification of the invasion plasmid antigen H (ipaH) gene by PCR amplification and DNA sequencing. Finally, we used a biochemical test and a serological assay to differentiate between Shigella and EIEC. Among the 93 water samples from nine reservoirs and one watershed, 76 (81.7%) water samples of culture plates had candidate colonies of Shigella and EIEC and 5 water samples were positive (5.4%) for a Shigella- and EIEC-specific polymerase chain reaction targeting the ipaH gene. Guided by the molecular method, the biochemical test, and the serological assay, 11 ipaH gene-positive isolates from 5 water samples were all identified as EIEC.

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Citations

Nov 10, 2010·Archives of Microbiology·Y Yamazaki, A Fukasawa
Feb 14, 2012·Environmental Science and Pollution Research International·Shih-Wei HuangYi-Chou Chiu
Apr 7, 2011·Enfermedades infecciosas y microbiología clínica·M Aurora Echeita SarrionandiaCristina Simón Baamonde
Feb 22, 2011·Journal of Applied Microbiology·M PavlovicI Huber
May 4, 2016·Environmental Science and Pollution Research International·Akebe Luther King AbiaMaggy Ndombo Benteke Momba
Jun 10, 2017·Archives of Microbiology·Shih-Chun YangJia-You Fang
Nov 14, 2014·Current Opinion in Gastroenterology·Ila F N LimaAldo A M Lima
Apr 1, 2020·Acta Microbiologica Et Immunologica Hungarica·Fatima Bachir HalimehJean-Marc Rolain

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