Differentiation and species identification of yeasts using PCR

International Journal of Systematic Bacteriology
M de Barros LopesP Langridge

Abstract

A PCR-based method has been developed that permits both intraspecies differentiation and species identification of yeast isolates. Oligonucleotide primers that are complementary to intron splice sites were used to produce PCR fingerprints that display polymorphisms between different species of indigenous wine yeasts. Although polymorphisms existed between isolates of the same species, the banding patterns shared several amplification products that allowed species identification. Importantly, the method was able to distinguish between species of the closely related Saccharomyces sensu stricto yeasts. In two cases where isolates could not be positively identified there was discrepancy between the phenetic and phylogenetic species concept. The method has applications in yeast ecological studies, enabling the rapid grouping of isolates with related genomes and the investigation of population dynamics of strains of the same species.

Citations

Apr 3, 2013·Applied Microbiology and Biotechnology·V BlättelH König
Sep 22, 2005·Journal of Industrial Microbiology & Biotechnology·Eurípedes Alves da Silva FilhoMarcos Antonio de Morais
Sep 4, 2010·Journal of Industrial Microbiology & Biotechnology·Angela XufreIsabel Spencer-Martins
Aug 8, 2012·Antonie van Leeuwenhoek·Feng-Li HuiChing-Fu Lee
Jul 6, 2000·Systematic and Applied Microbiology·E S NaumovE I Molina
Oct 20, 2005·Journal of Bioscience and Bioengineering·Sandra RainieriYoshinobu Kaneko
Jul 10, 2001·Letters in Applied Microbiology·A González TecheraC Gaggero
Nov 5, 2005·Applied and Environmental Microbiology·P MartorellM T Fernández-Espinar
Oct 7, 2004·Canadian Journal of Microbiology·Juliana L R FiettoIeso M Castro
Dec 10, 1999·Canadian Journal of Microbiology·P ValenteO Leoncini
Jan 8, 2013·International Journal of Food Microbiology·Natália O P MacielCarlos A Rosa
Sep 11, 2004·Journal of Applied Microbiology·N HierroJ M Guillamón
Jun 15, 2013·Yeast·Ana Teresa B F AntonangeloDébora Colombi
Feb 17, 2006·International Journal of Food Microbiology·Maristela de Araújo VicenteRogelio Lopes Brandão
Jan 20, 2007·FEMS Yeast Research·Chris D CurtinMiguel A de Barros Lopes
May 3, 2016·Animal : an International Journal of Animal Bioscience·E Vargas-Bello-PérezJ Romero
Jul 23, 2013·Fungal Genetics and Biology : FG & B·Camila G MoraisCarlos A Rosa
Sep 21, 2016·Fungal Biology·Franciele Pereira CamargoAndré Cordeiro Alves Dos Santos
Sep 1, 2006·Food Microbiology·Belén Suárez VallesRoberto Rodríguez Madrera
Jan 24, 2007·Journal of Applied Microbiology·A T de Souza LiberalM A de Morais
May 16, 2019·Microorganisms·Ricardo Franco-DuarteCélia F Rodrigues
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·Solange C CarreiroCarlos A Rosa
Jan 20, 2005·International Journal of Systematic and Evolutionary Microbiology·Raphael S PimentaCarlos A Rosa
Jan 16, 2007·International Journal of Systematic and Evolutionary Microbiology·Carlos A RosaPaula B Morais
Mar 21, 2019·Memórias do Instituto Oswaldo Cruz·Natália Op MacielCarlos A Rosa
Jul 28, 2020·Journal of Food Science and Technology·Elsa BeyeneGary M Wessel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.