Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Stem Cell Research & Therapy
Jianling ChenJinfu Wang

Abstract

Inner ear hair cells as mechanoreceptors are extremely important for hearing. Defects in hair cells are a major cause of deafness. Induced pluripotent stem cells (iPSCs) are promising for regenerating inner ear hair cells and treating hearing loss. Here, we investigated migration, differentiation, and synaptic connections of transplanted otic epithelial progenitors (OEPs) derived from human iPSCs in mouse cochlea. Human urinary cells isolated from a healthy donor were reprogramed to form iPSCs that were induced to differentiate into OEPs and hair cell-like cells. Immunocytochemistry, electrophysiological examination, and scanning electron microscopy were used to examine characteristics of induced hair cell-like cells. OEP-derived hair cell-like cells were cocultured with spiral ganglion neurons (SGNs), and the markers of synaptic connections were detected using immunocytochemistry and transmission electron microscope. In vivo, OEPs derived from iPSCs were transplanted into the cochlea of mice by injection through the round window. Migration, differentiation, and synaptic connections of transplanted cells were also examined by thin cochlear sectioning and immunohistochemistry. The induced hair cell-like cells displayed typical m...Continue Reading

References

Jul 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Nishikawa, F Sasaki
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·C DalyR Llinás
Jan 12, 2002·The Journal of Biological Chemistry·Tiffany C WheelerLian Li
Feb 16, 2005·Nature Medicine·Masahiko IzumikawaYehoash Raphael
Nov 5, 2005·Neuron·Meredith LeMasurier, Peter G Gillespie
Nov 12, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Norio YamamotoTasuku Honjo
Jun 15, 2006·The Journal of Membrane Biology·R NouvianT Moser
May 29, 2007·Developmental Biology·Shinji TakebayashiTasuku Honjo
Dec 28, 2007·Molecular and Cellular Biology·Haydn M ProsserAllan Bradley
Apr 9, 2008·Stem Cells and Development·Tatyana A ProkhorovaMoustapha Kassem
Jun 11, 2008·Journal of Neuroscience Research·Masahiro MatsumotoJuichi Ito
Oct 22, 2008·Nature Biotechnology·Trond AasenJuan Carlos Izpisúa Belmonte
Nov 26, 2008·American Journal of Otolaryngology·Yong FuShusheng Gong
Mar 3, 2009·Molecular Neurobiology·George Zanazzi, Gary Matthews
Mar 31, 2009·Nature Neuroscience·Maryline BeurgAnthony J Ricci
Jul 25, 2009·Neuroreport·Koji NishimuraJuichi Ito
Aug 25, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Frank Schmitz
Oct 30, 2009·The Journal of Biological Chemistry·Fuxin ShiAlbert S B Edge
Jun 4, 2011·Journal of the American Society of Nephrology : JASN·Ting ZhouMiguel A Esteban
Nov 10, 2012·Nature Protocols·Ting ZhouMiguel A Esteban
Jan 17, 2013·Hearing Research·Edwin W RubelJennifer S Stone
Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Toru MiwaEiji Yumoto
Jun 19, 2013·PLoS Biology·Artur A IndzhykulianGregory I Frolenkov
Jan 8, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yong-Ho ParkYehoash Raphael
Feb 12, 2014·Stem Cells and Development·Mohammad RonaghiStefan Heller
May 9, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fuxin ShiAlbert S B Edge
Oct 10, 2014·Stem Cells Translational Medicine·Robert BarrettDhruv Sareen
Apr 16, 2015·Cell and Tissue Research·C Wichmann, T Moser

❮ Previous
Next ❯

Citations

Jun 23, 2019·Human Molecular Genetics·Ryotaro OmichiRichard J H Smith
Jan 30, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Thomas R Van De Water
Aug 1, 2020·Frontiers in Cellular Neuroscience·Lucy S FrenchLivia S Carvalho
Aug 4, 2020·World Journal of Stem Cells·María Beatriz Durán-Alonso
Jan 9, 2020·Journal of Applied Genetics·Rimsha FarooqMuhammad Mustafa
Jan 11, 2021·EBioMedicine·Christian WrobelTobias Moser
Jan 29, 2021·Journal of the Association for Research in Otolaryngology : JARO·Aida NourbakhshXue Z Liu
Mar 7, 2020·Cell Stem Cell·Arun SharmaClive N Svendsen
May 23, 2020·Stem Cell Reports·Pei-Ciao TangRick F Nelson
Mar 30, 2021·Current Protocols·Justine M Renauld, Martin L Basch
May 26, 2021·Journal of the American Academy of Audiology·Jenna E BergmanAdrien A Eshraghi
Jan 19, 2021·ACS Biomaterials Science & Engineering·Sevda PouraghaeiAlireza Moshaverinia
Apr 8, 2020·Molecular Therapy. Methods & Clinical Development·So-Young ChangMin Young Lee
May 6, 2021·Stem Cells·Majlinda LakoMiodrag Stojkovic

❮ Previous
Next ❯

Methods Mentioned

BETA
cochlear implants
transfection
PCR
scanning electron microscopy
transmission electronic microscopy

Software Mentioned

HEKA
Origin
OriginLab
Pulse
Clamfit
Primer Premier
Oligo

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.