Differentiation between acute skin rejection in allotransplantation and T-cell mediated skin inflammation based on gene expression analysis

BioMed Research International
Dolores WolframStefan Schneeberger

Abstract

Advances in microsurgical techniques and immunosuppressive medication have rendered transplantation of vascularized composite allografts possible, when autologous tissue is neither available nor sufficient for reconstruction. However, skin rejection and side effects of long-term immunosuppression still remain a major hurdle for wide adoption of this excellent reconstructive technique. Histopathologic changes during acute skin rejection in vascular composite allotransplantation often mimic inflammatory skin disorders and are hard to distinguish. Hence, the identification of diagnostic and therapeutic markers specific for skin rejection is of particular clinical need. Here we present novel markers allowing for early differentiation between rejection in hind limb allotransplantation and contact hypersensitivity. Assessment of Ccl7, Il18, and Il1b expression is most indicative of distinguishing skin rejection from skin inflammatory disorders. Gene expression levels varied significantly across skin types and regions, indicating localization specific mechanism of leukocyte migration and infiltration. Expression of Il12b, Il17a, and Il1b gene expression levels differed significantly between rejection and inflammation, independent of t...Continue Reading

References

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Citations

Mar 2, 2016·Transplant International : Official Journal of the European Society for Organ Transplantation·Fadi Issa
Feb 4, 2016·Transplant International : Official Journal of the European Society for Organ Transplantation·Michelle SchneiderLinda C Cendales
Apr 11, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Theresa HautzStefan Schneeberger
Dec 1, 2017·Current Opinion in Organ Transplantation·Joanna W EtraGerald Brandacher
Nov 28, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Riccardo SchweizerJan A Plock
May 16, 2019·Annals of Surgery·Joanna W EtraW P Andrew Lee
Dec 5, 2017·BioMed Research International·F Popa, A V Georgescu
Jan 14, 2021·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Calum HoneymanHenk Giele
Feb 25, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Franka MessnerGerald Brandacher

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Methods Mentioned

BETA
biopsies
PCR
Assay
PCA
biopsy

Software Mentioned

Genesis
7500 System SDS
R
SPSS

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