Differentiation between closely-related Impatiens spp. and regional biotypes of Impatiens glandulifera using a highly-simplified and inexpensive method for MALDI-TOF MS

Plant Methods
Michael A ReeveDaisuke Kurose

Abstract

Matrix-assisted laser-desorption and ionisation time-of-flight mass spectroscopy (MALDI-TOF MS) is a powerful tool for the characterisation and/or identification of protein-containing samples. Several MALDI-TOF MS sample-preparation methods are currently available but few of these are well suited to the analysis of plant material. We have recently developed a simple, rapid, and relatively-cheap method for MALDI-TOF MS that is applicable to plant material (in addition to microbial and insect material), and our aim in this study was to distinguish between closely-related plant species and/or between regional biotypes within an invasive weed species using this method with a view to optimising the selection of biological control agents that can be used for weed management. We have employed a combination of principal-component analysis and closest-relatedness diagrams derived from MALDI-TOF MS spectral-comparison data to discriminate between the closely-related Impatiens spp. Impatiens noli-tangere, Impatiens parviflora, Impatiens scabrida, Impatiens balsamina, and two regional biotypes of the invasive weed Impatiens glandulifera. We have also developed a method for sample discrimination based upon comparison between blind-test MALD...Continue Reading

Citations

Mar 13, 2020·Biology Methods and Protocols·Michael A ReeveThelma S Caine
May 13, 2020·Biology Methods and Protocols·Michael A Reeve, Kathryn M Pollard
May 13, 2020·Biology Methods and Protocols·Michael A Reeve, Denise Bachmann

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Methods Mentioned

BETA
PCA

Software Mentioned

Bruker Online Client
MALDI Biotyper RTC

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