PMID: 8587802Jan 1, 1996Paper

Differentiation between culture-derived insect stages of T. brucei, T. vivax, T. congolense and T. simiae using a monoclonal antibody-based dot-ELISA

Parasitology
K M BosompemV M Nantulya

Abstract

A sensitive and specific nitrocellulose (NC) membrane-based dot-ELISA, utilizing a panel of monoclonal antibodies (mAbs), was developed for differentiation between in vitro-derived procyclic forms of Trypanosoma brucei, T. congolense and T. simiae, and epimastigotes of T. vivax. Trypanosomes in suspension were applied onto NC membrane in dots and probed with unlabelled trypanosome species-specific mAbs. Bound mAb was revealed by enzyme labelled anti-mouse IgG and precipitable chromogenic substrate. The assay detected the aforementioned trypanosome species in both single and artificially mixed preparations. Ten T. brucei, 4 T. vivax, 7 T. congolense and 3 T. simiae procyclic stocks and clones from different geographical areas were tested and identified using the specific mAbs in the dot-ELISA which had a specificity of 100%. Some of the T. brucei, T. congolense and Nannomonas-specific mAbs could detect as few as 10 trypanosomes/dot, whilst 1 T. vivax mAb was able to detect a minimum of 100 trypanosomes/dot in monospecies preparations. A concentration of 1 x 10(4) trypanosomes/microliters/dot was eventually determined as ideal for testing in the dot-ELISA. Antigen dots stored at 4 degrees C under desiccated conditions did not sho...Continue Reading

References

Jan 1, 1979·Journal of Immunological Methods·S M BurtL J Kricka
Dec 1, 1979·Social Science & Medicine. Medical Anthropology·J Ford
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·M MurrayW I McIntyre
Jan 1, 1989·Advances in Parasitology·P R Gardiner
Oct 24, 1985·Journal of Immunological Methods·S KumarG P Talwar
Jul 1, 1986·Journal of Clinical Microbiology·T M Lin, S P Halbert
Sep 1, 1988·Veterinary Parasitology·M G Pappas
Oct 1, 1987·Molecular and Biochemical Parasitology·T W PearsonL Jenni
Jan 1, 1986·Methods in Enzymology·A Boeyé
Sep 4, 1984·Journal of Immunological Methods·H Towbin, J Gordon
Nov 1, 1984·The American Journal of Tropical Medicine and Hygiene·M G PappasW T Hockmeyer
Jun 1, 1983·Annals of Tropical Medicine and Parasitology·L H Otieno
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Dec 1, 1959·Annals of Tropical Medicine and Parasitology·W TRAGER

❮ Previous
Next ❯

Citations

Feb 17, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·K M BosompemS Kojima
Jul 14, 2000·Journal of Virological Methods·L LlamesE Gomez-Lucia
Jun 15, 2010·Trends in Parasitology·John C EnyaruDaniel K Masiga
Aug 12, 2004·Tropical Medicine & International Health : TM & IH·K M BosompemN Ohta

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.