PMID: 8598269Jan 1, 1996Paper

Differentiation between human and ovine isolates of Bordetella parapertussis using pulsed-field gel electrophoresis

FEMS Microbiology Letters
J F PorterW Donachie

Abstract

The genetic relatedness of 18 human and 29 ovine isolates of Bordetella parapertussis was examined by macrorestriction digestion of DNA with the rarely cutting enzyme XbaI and resolution by pulsed-field gel electrophoresis. There was clear separation of human and ovine isolates and variation within host types. The human isolates were separated into three types as were the 24 Scottish ovine isolates. Species-specific bands were observed with the human isolates at 114, 134, 166, 213, 346 and 372 kb. No species-specific bands were found in the B. parapertussis ovine isolates. Isolates of B. parapertussis recovered from sheep in New Zealand gave a further two DNA banding patterns which were clearly different from the Scottish ovine and the human isolates. These results indicate that human and ovine isolates of B. parapertussis are genetically distinct and that variation exists within isolates from the same host species. Pulsed-field gel electrophoresis therefore appears to be a powerful discriminatory tool for the classification of B. parapertussis.

References

Apr 1, 1986·Journal of Bacteriology·J M MusserR K Selander
Oct 1, 1993·International Journal of Systematic Bacteriology·M N Khattak, R C Matthews
Oct 1, 1987·New Zealand Veterinary Journal·L C CullinaneB W Manktelow

❮ Previous
Next ❯

Citations

Apr 22, 2004·Nature Reviews. Microbiology·Andrew PrestonDuncan J Maskell
May 1, 1999·The Pediatric Infectious Disease Journal·J E Hoppe
Jun 18, 2002·Infection and Immunity·Ulrich HeiningerEric T Harvill
Jul 6, 2005·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Andrew Preston
Nov 3, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Frits R Mooi
Mar 13, 2002·The Journal of Biological Chemistry·Charles R SweetChristian R H Raetz
Jan 6, 2011·FEMS Microbiology Reviews·Daan de GouwFrits R Mooi
Jul 5, 2016·Frontiers in Pediatrics·Manuela Zlamy
Oct 25, 2007·Journal of Endotoxin Research·Iain MacArthurAndrew Preston
Jun 27, 2006·Vector Borne and Zoonotic Diseases·Abdoulaye NgomAyayi Justin Ayih-Akakpo
Jun 11, 2008·The Journal of Infectious Diseases·Shokrollah ElahiVolker Gerdts
Oct 16, 2019·Journal of Medical Microbiology·Kazunari KamachiMineo Watanabe
Jul 23, 1999·Infection and Immunity·A PrestonD J Maskell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.