Differentiation between human red cells of Pk and p blood types using Pseudomonas aeruginosa PA-I lectin

Transfusion
Dvora SudakevitzNechama Gilboa-Garber

Abstract

The red cells of almost all human beings bear P antigen. Type P1 cells (around 75% of the population) contain P1 antigen in addition to P, and type P2 cells (around 25% of the population) contain only P. The red cells of only a few individuals are devoid of P: these cells may be of either Pk-positive (P1k and P2k[Pk]) or p type (the latter lack all the above-described antigens of the P system). Differentiation between them is of clinical importance, but there is a shortage of specific reagents. This article offers reliable means for differentiation. Agglutination of washed, papain-treated red cells of all the P types by PA-1 and soybean lectins and adsorption of the lectins onto the red cells were examined. PA-1 strongly agglutinated papain-treated red cells. Examination of its interactions with red cells having different P system antigens revealed that Pk (both P1k and P2k) red cells of O, A, and B blood types were agglutinated significantly faster than p red cells. The agglutination intensities of Pk red cells of types O and A (most people) was considerably stronger than those of p red cells. P1 and P2 type red cell agglutination was intermediate (P1>P2). Adsorption tests with all the red cells, exhibited the same order of PA...Continue Reading

Citations

Dec 26, 2001·FEMS Immunology and Medical Microbiology·B Lerrer, N Gilboa-Garber
Feb 2, 2002·Canadian Journal of Microbiology·B Lerrer, N Gilboa-Garber
Apr 3, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Kuang-Che KuoSan-Nan Yang
Jun 19, 2015·Clinical Microbiology Reviews·Laura Cooling
Dec 10, 2002·The Journal of the American Academy of Orthopaedic Surgeons·E Michael Keating, John B Meding
Jul 4, 2009·Journal of Agricultural and Food Chemistry·Keren D Zinger-Yosovich, Nechama Gilboa-Garber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.